RRC ID |
67947
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著者 |
Sawada H, Yamamoto K, Yamaguchi A, Yamada L, Higuchi A, Nukaya H, Fukuoka M, Sakuma T, Yamamoto T, Sasakura Y, Shirae-Kurabayashi M.
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タイトル |
Three multi-allelic gene pairs are responsible for self-sterility in the ascidian Ciona intestinalis.
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ジャーナル |
Sci Rep
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Abstract |
Many hermaphroditic organisms possess a self-incompatibility system to avoid inbreeding. Although the mechanisms of self-incompatibility in flowering plants are well known, little is known about the mechanisms of self-sterility in hermaphroditic marine invertebrates. Ascidians are hermaphroditic sessile marine invertebrates that release sperm and eggs into the surrounding seawater. Several species, including Ciona intestinalis type A (Ciona robusta), exhibit strict self-sterility. In a previous study, we found that the candidate genes responsible for self-sterility in Ciona reside in chromosome 2q (locus A) and chromosome 7q (locus B). Two pairs of multi-allelic genes, named s(sperm)-Themis-A and v(vitelline-coat)-Themis-A in locus A and s-Themis-B and v-Themis-B in locus B, are responsible for self-sterility. In this study, we identified a third multi-allelic gene pair, s-Themis-B2 and v-Themis-B2, within locus B that is also involved in this system. Genetic analysis revealed that the haplotypes of s/v-Themis-A, s/v-Themis-B and s/v-Themis-B2 play essential roles in self-sterility. When three haplotypes were matched between s-Themis and v-Themis, fertilization never occurred even in nonself crossing. Interestingly, gene targeting of either s/v-Themis-B/B2 or s/v-Themis-A by genome editing enabled self-fertilization. These results indicate that s/v-Themis-A, -B and -B2 are S-determinant genes responsible for self-sterility in the ascidian C. intestinalis type A.
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巻・号 |
10(1)
|
ページ |
2514
|
公開日 |
2020-2-13
|
DOI |
10.1038/s41598-020-59147-4
|
PII |
10.1038/s41598-020-59147-4
|
PMID |
32054881
|
PMC |
PMC7018956
|
MeSH |
Alleles
Animals
Ciona intestinalis / genetics*
Ciona intestinalis / physiology*
Female
Hermaphroditic Organisms / genetics
Hermaphroditic Organisms / physiology
Infertility
Male
Self-Fertilization
|
IF |
3.998
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リソース情報 |
カタユウレイボヤ・(ニッポンウミシダ) |
wild C. int. |