RRC ID 67982
Author Iwamoto N, Ito K, Kakuchi J, Yoshioka T.
Title Quenching of nitric oxide by an oral carbonaceous adsorbent.
Journal Jpn J Pharmacol
Abstract The ability of carbonaceous particles (AST-120), originally developed as an enteral adsorbent of uremic toxins, to quench nitric oxide (NO) was tested. NO in solutions prepared by two methods [NO gas bubbling and NO generating system, i.e., decomposition of 1-hydroxy-2-oxo-3-(aminopropyl)-3-isopropyl-1-triazene] were determined by a NO-specific reduction of carboxy-2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide using an electron paramagnetic resonance spectrometry. NO concentrations were less in samples containing increasing concentrations of AST-120. In a separate study, nitrite concentrations in lipopolysaccharide-treated RAW264 cells were significantly less in incubation medium containing AST-120. Thus, AST-120 may be applicable as an enteral anti-NO agent.
Volume 74(2)
Pages 213-6
Published 1997-6-1
DOI 10.1254/jjp.74.213
PMID 9243331
MeSH Adsorption Carbon / pharmacology* Cell Line Electron Spin Resonance Spectroscopy Humans In Vitro Techniques Macrophages / metabolism Nitric Oxide / chemistry Nitric Oxide / metabolism* Oxides / pharmacology*
Human and Animal Cells RAW 264(RCB0535)