RRC ID 67992
Author Motohashi H, Ohta J, Engel JD, Yamamoto M.
Title A core region of the mafK gene IN promoter directs neurone-specific transcription in vivo.
Journal Genes Cells
Abstract BACKGROUND:MafK serves as a required subunit of erythroid transcription factor NF-E2 and also functions with various heterodimeric CNC family proteins. MafK expression begins in early mesoderm and is observed in mesenchymal and haematopoietic cells, as well as in neurones during mouse development. In mesodermal descendants, MafK mRNA begins with a distal first exon (called IM), whereas the mRNA in neurones begins with a proximal first exon (IN).
RESULTS:To elucidate the mechanisms that underlie the tissue-specific transcription of the mafK gene, and to gain insights into the functions of MafK during neural development, we analysed the activity of the mafK IN promoter. A detailed investigation of mafK expression in the embryonic spinal cord revealed that IN-initiated mRNA is expressed in the ventral side of the spinal cord. Transient transfection analysis of reporter plasmids bearing the IN promoter and upstream regions revealed that the 'core' region of this promoter (nt -67 to -9) is active and that its integrity is crucial for this activity. The core region was also capable of directing the tissue-specific transcription of a reporter gene in neural cells of the spinal cord in transgenic mice in vivo.
CONCLUSION:These results demonstrate that the specific expression of mafK in neural cells is determined, at least in part, by the core region of the IN promoter.
Volume 3(10)
Pages 671-84
Published 1998-10-1
DOI 10.1046/j.1365-2443.1998.00222.x
PMID 9893024
MeSH Animals Base Sequence Cell Line Exons Gene Expression Regulation, Developmental Immunohistochemistry In Situ Hybridization Lac Operon Luciferases / metabolism MafK Transcription Factor Mice Mice, Transgenic Molecular Sequence Data Neurons / metabolism* Nuclear Proteins / genetics* Nuclear Proteins / metabolism PC12 Cells Promoter Regions, Genetic / genetics* RNA, Messenger / metabolism Rats Reverse Transcriptase Polymerase Chain Reaction Spinal Cord / embryology Spinal Cord / metabolism Transcription, Genetic*
IF 1.655
Human and Animal Cells C-1300(RCB0283)