論文 - 詳細
| RRC ID | 67996 |
|---|---|
| 著者 | Numazawa S, Yamada H, Furusho A, Nakahara T, Oguro T, Yoshida T. |
| タイトル | Cooperative induction of c-fos and heme oxygenase gene products under oxidative stress in human fibroblastic cells. |
| ジャーナル | Exp Cell Res |
| Abstract |
Heme oxygenase-1 is a stress responsive enzyme and implicated in a protective function of cellular damage. We investigated cellular events leading to the heme oxygenase-1 gene expression induced by sublethal concentrations of glutathione depletors, phorone and diethyl maleate, in human fibroblastic cells. Accumulation of heme oxygenase-1 mRNA by glutathione depletors was canceled by simultaneous treatment with cycloheximide, an inhibitor of protein synthesis; however, the inhibitory effect decreased when the inhibitor was added 30 min later. Among the inducible early response genes, the c-fos expression was significantly elevated with a peak at 30 min after the agents. Accumulation of heme oxygenase-1 and c-fos transcripts was abrogated in cells pretreated with 1,4-diazabicyclo[2.2.2]octane, an oxygen-free radical quencher. Decrease in glutathione levels preferentially activated extracellular-signal regulated kinases rather than other stress-activated protein kinases such as c-Jun N-terminal kinases and p38 MAP kinase. Pretreatment of cells with PD 98059, an inhibitor of the extracellular-signal regulated kinase cascade, or the c-fos antisense oligodeoxynucleotide inhibited the heme oxygenase-1 induction elicited by glutathione depletion. These observations indicated that c-Fos protein plays a role in heme oxygenase-1 gene expression induced by glutathione depletion-mediated oxidative stress in human fibroblasts. |
| 巻・号 | 237(2) |
| ページ | 434-44 |
| 公開日 | 1997-12-15 |
| DOI | 10.1006/excr.1997.3825 |
| PII | S0014-4827(97)93825-8 |
| PMID | 9434639 |
| MeSH | Calcium-Calmodulin-Dependent Protein Kinases / metabolism Cell Division / drug effects Cell Line Enzyme Induction / drug effects Fibroblasts / drug effects Fibroblasts / metabolism* Flavonoids / pharmacology Gene Expression Regulation, Enzymologic / drug effects Genes, Immediate-Early Glutathione / metabolism Heme Oxygenase (Decyclizing) / biosynthesis* Humans Ketones / pharmacology Maleates / pharmacology Mitogen-Activated Protein Kinases / metabolism Nerve Tissue Proteins / metabolism Oxidative Stress / drug effects Oxidative Stress / genetics* Proto-Oncogene Proteins c-fos / biosynthesis* RNA, Messenger / genetics |
| IF | 3.383 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | WI-38(RCB0702) |