| RRC ID |
67
|
| Author |
Takeuchi K, Sereemaspun A, Inagaki T, Hakamata Y, Kaneko T, Murakami T, Takahashi M, Kobayashi E, Ookawara S.
|
| Title |
Morphologic characterization of green fluorescent protein in embryonic, neonatal, and adult transgenic rats.
|
| Journal |
Anat Rec A Discov Mol Cell Evol Biol
|
| Abstract |
Transgenic (Tg) animals with reporter genes are useful models in which to study cell lineage and the process of differentiation into tissues. We developed the green fluorescent protein (GFP)-Tg rat, which is more suitable for transplantation and stem cell research because it is larger than mice. We found that marker gene expression was dependent on each organ and developmental stage. In this study we describe GFP expression in various tissues from embryonic, neonatal, and adult animals. GFP expression in brain, lung, liver, and islet tissues was restricted to early developmental stages, but it was continuously strong in the exocrine pancreas, kidney, and cardiac and skeletal muscles. The CAG promoter that was presumed to induce ubiquitous protein expression might be responsible for the differences in expression.
|
| Volume |
274(2)
|
| Pages |
883-6
|
| Published |
2003-10-1
|
| DOI |
10.1002/ar.a.10111
|
| PMID |
12973712
|
| MeSH |
Animals
Animals, Genetically Modified* / metabolism
Animals, Newborn / metabolism*
Embryo, Mammalian / metabolism*
Gene Expression
Genes, Reporter
Genetic Markers
Green Fluorescent Proteins
Indicators and Reagents*
Luminescent Proteins / genetics
Luminescent Proteins / metabolism*
Organogenesis
Promoter Regions, Genetic
Rats
Rats, Wistar
Tissue Distribution
|
| Times Cited |
21
|
|
WOS Category
|
ANATOMY & MORPHOLOGY
|
| Resource |
| Rats |
W-Tg(CAG-GFP)184Ys(strainID=525) |
