論文 - 詳細
| RRC ID | 6805 |
|---|---|
| 著者 | Hashimoto O, Sekiyama K, Matsuo T, Hasegawa Y. |
| タイトル | Implication of activin E in glucose metabolism: transcriptional regulation of the inhibin/activin betaE subunit gene in the liver. |
| ジャーナル | Life Sci |
| Abstract |
AIMS:Activin E is a newly identified member of the transforming growth factor-beta superfamily. To assess the role of activin E in glucose/energy metabolism, we investigated the transcriptional regulation of activin E in the liver. MAIN METHODS:Northern blotting, Western blotting, quantitative real-time polymerase chain reaction (PCR), reporter assays and chromatin immunoprecipitation assays were used in this study. KEY FINDINGS:Insulin up-regulated activin E expression at the mRNA and protein level in HepG2 cells. Reporter assays revealed that the putative, functional, promoter sequence of human activin E gene was responsible for the effect of insulin. Mutational analysis of the promoter revealed that CCAAT/enhancer-binding proteins (C/EBPs) play a key role in regulating activin E expression and in the stimulatory effect of insulin on activin E transcription. Chromatin immunoprecipitation assays revealed that the C/EBPs can bind to the activin E promoter in HepG2 cells. The expression of activin E mRNA was up-regulated in the liver of diet-induced obese mice. SIGNIFICANCE:These observations suggest that activin E plays a pathophysiological role in glucose metabolism. |
| 巻・号 | 85(13-14) |
| ページ | 534-40 |
| 公開日 | 2009-9-23 |
| DOI | 10.1016/j.lfs.2009.08.007 |
| PII | S0024-3205(09)00335-X |
| PMID | 19703474 |
| MeSH | Activins / genetics Activins / metabolism* Animals Blotting, Northern Blotting, Western Carbohydrate Metabolism / genetics* Cell Line, Tumor Gene Expression Regulation* Glucose / metabolism* Hepatocytes / drug effects Humans Hypoglycemic Agents / pharmacology Inhibin-beta Subunits / drug effects* Inhibin-beta Subunits / genetics Inhibin-beta Subunits / metabolism Insulin / pharmacology Liver / metabolism* Male Mice Mice, Inbred C57BL Promoter Regions, Genetic RNA, Messenger / biosynthesis RNA, Messenger / drug effects |
| IF | 3.647 |
| 引用数 | 13 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | |