RRC ID 68062
Author Moriuchi T, Hirose F.
Title SUMOylation of RepoMan during late telophase regulates dephosphorylation of lamin A.
Journal J Cell Sci
Abstract Dephosphorylation of lamin A, which triggers nuclear lamina reconstitution, is crucial for the completion of mitosis. However, the specific phosphatase and regulatory mechanism that allow timely lamin A dephosphorylation remain unclear. Here, we report that RepoMan (also known as CDCA2), a regulatory subunit of protein phosphatase 1γ (PP1γ) is transiently modified with SUMO-2 at K762 during late telophase. SUMOylation of RepoMan markedly enhanced its binding affinity with lamin A. Moreover, SUMOylated RepoMan contributes to lamin A recruitment to telophase chromosomes and dephosphorylation of the mitotic lamin A phosphorylation. Expression of a SUMO-2 mutant that has a defective interaction with the SUMO-interacting motif (SIM) resulted in failure of the lamin A and RepoMan association, along with abrogation of lamin A dephosphorylation and subsequent nuclear lamina formation. These findings strongly suggest that RepoMan recruits lamin A through SUMO-SIM interaction. Thus, transient SUMOylation of RepoMan plays an important role in the spatiotemporal regulation of lamin A dephosphorylation and the subsequent nuclear lamina formation at the end of mitosis.
Volume 134(17)
Published 2021-9-1
DOI 10.1242/jcs.247171
PII 271831
PMID 34387316
PMC PMC8445599
MeSH Lamin Type A* / genetics Lamin Type A* / metabolism Mitosis Nuclear Proteins / genetics Nuclear Proteins / metabolism Sumoylation* Telophase
IF 4.573
DNA material CS-RfA-EG (RDB04391)