論文 - 詳細
| RRC ID | 68062 |
|---|---|
| 著者 | Moriuchi T, Hirose F. |
| タイトル | SUMOylation of RepoMan during late telophase regulates dephosphorylation of lamin A. |
| ジャーナル | J Cell Sci |
| Abstract |
Dephosphorylation of lamin A, which triggers nuclear lamina reconstitution, is crucial for the completion of mitosis. However, the specific phosphatase and regulatory mechanism that allow timely lamin A dephosphorylation remain unclear. Here, we report that RepoMan (also known as CDCA2), a regulatory subunit of protein phosphatase 1γ (PP1γ) is transiently modified with SUMO-2 at K762 during late telophase. SUMOylation of RepoMan markedly enhanced its binding affinity with lamin A. Moreover, SUMOylated RepoMan contributes to lamin A recruitment to telophase chromosomes and dephosphorylation of the mitotic lamin A phosphorylation. Expression of a SUMO-2 mutant that has a defective interaction with the SUMO-interacting motif (SIM) resulted in failure of the lamin A and RepoMan association, along with abrogation of lamin A dephosphorylation and subsequent nuclear lamina formation. These findings strongly suggest that RepoMan recruits lamin A through SUMO-SIM interaction. Thus, transient SUMOylation of RepoMan plays an important role in the spatiotemporal regulation of lamin A dephosphorylation and the subsequent nuclear lamina formation at the end of mitosis. |
| 巻・号 | 134(17) |
| 公開日 | 2021-9-1 |
| DOI | 10.1242/jcs.247171 |
| PII | 271831 |
| PMID | 34387316 |
| PMC | PMC8445599 |
| MeSH | Lamin Type A* / genetics Lamin Type A* / metabolism Mitosis Nuclear Proteins / genetics Nuclear Proteins / metabolism Sumoylation* Telophase |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CS-RfA-EG (RDB04391) |