論文 - 詳細
| RRC ID | 68066 |
|---|---|
| 著者 | Takada N, Takatsu H, Miyano R, Nakayama K, Shin HW. |
| タイトル | ATP11C mutation is responsible for the defect in phosphatidylserine uptake in UPS-1 cells. |
| ジャーナル | J Lipid Res |
| Abstract |
Type IV P-type ATPases (P4-ATPases) translocate phospholipids from the exoplasmic to the cytoplasmic leaflets of cellular membranes. We and others previously showed that ATP11C, a member of the P4-ATPases, translocates phosphatidylserine (PS) at the plasma membrane. Twenty years ago, the UPS-1 (uptake of fluorescent PS analogs) cell line was isolated from mutagenized Chinese hamster ovary (CHO)-K1 cells with a defect in nonendocytic uptake of nitrobenzoxadiazole PS. Due to its defect in PS uptake, the UPS-1 cell line has been used in an assay for PS-flipping activity; however, the gene(s) responsible for the defect have not been identified to date. Here, we found that the mRNA level of ATP11C was dramatically reduced in UPS-1 cells relative to parental CHO-K1 cells. By contrast, the level of ATP11A, another PS-flipping P4-ATPase at the plasma membrane, or CDC50A, which is essential for delivery of most P4-ATPases to the plasma membrane, was not affected in UPS-1 cells. Importantly, we identified a nonsense mutation in the ATP11C gene in UPS-1 cells, indicating that the intact ATP11C protein is not expressed. Moreover, exogenous expression of ATP11C can restore PS uptake in UPS-1 cells. These results indicate that lack of the functional ATP11C protein is responsible for the defect in PS uptake in UPS-1 cells and ATP11C is crucial for PS flipping in CHO-K1 cells. |
| 巻・号 | 56(11) |
| ページ | 2151-7 |
| 公開日 | 2015-11-1 |
| DOI | 10.1194/jlr.M062547 |
| PII | S0022-2275(20)35484-5 |
| PMID | 26420878 |
| PMC | PMC4617402 |
| MeSH | Adenosine Triphosphatases / physiology* Animals CHO Cells Cell Membrane / enzymology Cricetinae Cricetulus Humans Membrane Transport Proteins / physiology* Phosphatidylserines / metabolism* |
| IF | 4.483 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | UPS-1(RCB4451) |