論文 - 詳細
| RRC ID | 68131 |
|---|---|
| 著者 | Abe T, Uosaki H, Shibata H, Hara H, Sarentonglaga B, Nagao Y, Hanazono Y. |
| タイトル | Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells. |
| ジャーナル | Exp Hematol |
| Abstract |
We report that a sheep fetal liver provides a microenvironment for generating hematopoietic cells with long-term engrafting capacity and multilineage differentiation potential from human induced pluripotent stem cell (iPSC)-derived hemogenic endothelial cells (HEs). Despite the promise of iPSCs for making any cell types, generating hematopoietic stem and progenitor cells (HSPCs) is still a challenge. We hypothesized that the hematopoietic microenvironment, which exists in fetal liver but is lacking in vitro, turns iPSC-HEs into HSPCs. To test this, we transplanted CD45-negative iPSC-HEs into fetal sheep liver, in which HSPCs first grow. Within 2 months, the transplanted cells became CD45 positive and differentiated into multilineage blood cells in the fetal liver. Then, CD45-positive cells translocated to the bone marrow and were maintained there for 3 years with the capability of multilineage differentiation, indicating that hematopoietic cells with long-term engraftment potential were generated. Moreover, human hematopoietic cells were temporally enriched by xenogeneic donor-lymphocyte infusion into the sheep. This study could serve as a foundation to generate HSPCs from iPSCs. |
| 巻・号 | 95 |
| ページ | 46-57.e8 |
| 公開日 | 2021-3-1 |
| DOI | 10.1016/j.exphem.2020.12.006 |
| PII | S0301-472X(20)30695-0 |
| PMID | 33395577 |
| MeSH | Animals Bone Marrow Cells / cytology Cell Differentiation Cell Lineage Cell Movement Cellular Microenvironment Colony-Forming Units Assay Cord Blood Stem Cell Transplantation Female Genetic Techniques Graft Survival Hemangioblasts / cytology Hematopoietic Stem Cells / cytology* Heterografts Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / transplantation* Leukocyte Common Antigens / analysis Liver / embryology Lymphocyte Subsets Pregnancy Sheep / embryology* Species Specificity |
| IF | 2.82 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 409B2(HPS0076) 454E2(HPS0077) |