RRC ID 68136
Author Izumiya M, Haniu M, Ueda K, Ishida H, Ma C, Ideta H, Sobajima A, Ueshiba K, Uemura T, Saito N, Haniu H.
Title Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media.
Journal Int J Mol Sci
Abstract Many biomaterials have been evaluated using cultured cells. In particular, osteoblast-like cells are often used to evaluate the osteocompatibility, hard-tissue-regeneration, osteoconductive, and osteoinductive characteristics of biomaterials. However, the evaluation of biomaterial osteogenesis-inducing capacity using osteoblast-like cells is not standardized; instead, it is performed under laboratory-specific culture conditions with different culture media. However, the effect of different media conditions on bone formation has not been investigated. Here, we aimed to evaluate the osteogenesis of MC3T3-E1 cells, one of the most commonly used osteoblast-like cell lines for osteogenesis evaluation, and assayed cell proliferation, alkaline phosphatase activity, expression of osteoblast markers, and calcification under varying culture media conditions. Furthermore, the various media conditions were tested in uncoated plates and plates coated with collagen type I and poly-L-lysine, highly biocompatible molecules commonly used as pseudobiomaterials. We found that the type of base medium, the presence or absence of vitamin C, and the freshness of the medium may affect biomaterial regeneration. We posit that an in vitro model that recapitulates in vivo bone formation should be established before evaluating biomaterials.
Volume 22(14)
Published 2021-7-20
DOI 10.3390/ijms22147752
PII ijms22147752
PMID 34299372
PMC PMC8304275
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Animals Ascorbic Acid / pharmacology Biocompatible Materials / pharmacology Biomarkers / metabolism Cell Line Cell Proliferation / drug effects Collagen Type I / metabolism Culture Media, Conditioned / pharmacology* Mice Osteoblasts / drug effects Osteoblasts / metabolism Osteogenesis / drug effects*
IF 4.556
Human and Animal Cells MC3T3-E1(RCB1126)