論文 - 詳細
| RRC ID | 6814 |
|---|---|
| 著者 | Miyata K, Gouda N, Takemoto H, Oba M, Lee Y, Koyama H, Yamasaki Y, Itaka K, Nishiyama N, Kataoka K. |
| タイトル | Enhanced transfection with silica-coated polyplexes loading plasmid DNA. |
| ジャーナル | Biomaterials |
| Abstract |
Silica-coating of positively charged polyplexes was demonstrated through silicic acid condensation to improve the polyplexes for enhanced complex stability and transfection efficiency. Silicic acid was efficiently condensed by polycations to form a silica network in the polyplex through electrostatic interaction and hydrogen bonding. The silica-coated (SC) polyplexes had an anionic surface charge of -20 mV and were 10-20 nm larger in size compared to the non-silica-coated control (+33.4 mV, 106 nm). Silica-coating significantly improved the polyplex stability against both dissociations by counter polyanion exchange and aggregation by salt. The silica network was dissolved to form silicic acid by removing free silicic acid based on the equilibrium, SiO(2) + 2H(2)O right arrow over left arrow Si(OH)(4). Indeed, dialysis of the SC polyplex solution against excess silica-free buffer permitted plasmid DNA release from the silica-coated polyplex, indicating the reversible nature of the silica-layer. The SC polyplex achieved significantly higher transfection efficiency without serious cytotoxicity compared to the polyplex without silica-coating. Detailed examinations of transfection using SC polyplexes revealed that the enhanced transfection efficiency was because of facilitated endosomal escape, possibly due to the protonation of the silica in acidic endosomal compartments. These findings demonstrate the utility of the silica-coating technique for polyplex-mediated gene delivery. |
| 巻・号 | 31(17) |
| ページ | 4764-70 |
| 公開日 | 2010-6-1 |
| DOI | 10.1016/j.biomaterials.2010.02.033 |
| PII | S0142-9612(10)00262-0 |
| PMID | 20304483 |
| MeSH | Cell Line, Tumor Cell Survival / drug effects DNA / genetics* Electrophoresis, Agar Gel Flow Cytometry Humans Microscopy, Confocal Plasmids / genetics* Polymers / adverse effects Polymers / chemistry* Polymers / pharmacology Silicon Dioxide / chemistry* Transfection / methods* |
| IF | 10.317 |
| 引用数 | 27 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7(RCB1366) |