| RRC ID |
6814
|
| 著者 |
Miyata K, Gouda N, Takemoto H, Oba M, Lee Y, Koyama H, Yamasaki Y, Itaka K, Nishiyama N, Kataoka K.
|
| タイトル |
Enhanced transfection with silica-coated polyplexes loading plasmid DNA.
|
| ジャーナル |
Biomaterials
|
| Abstract |
Silica-coating of positively charged polyplexes was demonstrated through silicic acid condensation to improve the polyplexes for enhanced complex stability and transfection efficiency. Silicic acid was efficiently condensed by polycations to form a silica network in the polyplex through electrostatic interaction and hydrogen bonding. The silica-coated (SC) polyplexes had an anionic surface charge of -20 mV and were 10-20 nm larger in size compared to the non-silica-coated control (+33.4 mV, 106 nm). Silica-coating significantly improved the polyplex stability against both dissociations by counter polyanion exchange and aggregation by salt. The silica network was dissolved to form silicic acid by removing free silicic acid based on the equilibrium, SiO(2) + 2H(2)O right arrow over left arrow Si(OH)(4). Indeed, dialysis of the SC polyplex solution against excess silica-free buffer permitted plasmid DNA release from the silica-coated polyplex, indicating the reversible nature of the silica-layer. The SC polyplex achieved significantly higher transfection efficiency without serious cytotoxicity compared to the polyplex without silica-coating. Detailed examinations of transfection using SC polyplexes revealed that the enhanced transfection efficiency was because of facilitated endosomal escape, possibly due to the protonation of the silica in acidic endosomal compartments. These findings demonstrate the utility of the silica-coating technique for polyplex-mediated gene delivery.
|
| 巻・号 |
31(17)
|
| ページ |
4764-70
|
| 公開日 |
2010-6-1
|
| DOI |
10.1016/j.biomaterials.2010.02.033
|
| PII |
S0142-9612(10)00262-0
|
| PMID |
20304483
|
| MeSH |
Cell Line, Tumor
Cell Survival / drug effects
DNA / genetics*
Electrophoresis, Agar Gel
Flow Cytometry
Humans
Microscopy, Confocal
Plasmids / genetics*
Polymers / adverse effects
Polymers / chemistry*
Polymers / pharmacology
Silicon Dioxide / chemistry*
Transfection / methods*
|
| IF |
10.317
|
| 引用数 |
27
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-7(RCB1366) |
