| RRC ID |
6816
|
| 著者 |
Nagamatsu Y, Okamura S, Saitou H, Akao T, Mizuki E.
|
| タイトル |
Three Cry toxins in two types from Bacillus thuringiensis strain M019 preferentially kill human hepatocyte cancer and uterus cervix cancer cells.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Bacillus thuringiensis strain M019, non-pathogenic to lepidopteran and dipteran insects, produces a parasporal inclusion that consists of three 84-kDa Cry proteins (CPs). CP78A and CP78B, which exhibit 83.5% amino acid identity, were new variants of the previously reported HeLa cell-killing protein (parasporin-1). CP84 was a novel CP showing low-level homology, of 21.9% (56.4% similarity), with the insecticidal Cry2 toxin. In vitro solubilization with dithiothreitol at pH 10.2 and limited hydrolysis with trypsin resulted in the removal of N-terminal portions of the CPs and their activation. The 70-kDa proteins (15- and 55-kDa fragments) from CP78A and CP78B and the 73-kDa protein (14- and 59-kDa fragments) from CP84 exhibited varying degrees of cytocidal activity preferentially toward human hepatocyte cancer HepG2 cells and uterus cervix cancer HeLa cells causing cell swelling or the formation of vacuoles in the cytoplasm. These toxins appeared to attack an identical target on human cells.
|
| 巻・号 |
74(3)
|
| ページ |
494-8
|
| 公開日 |
2010-1-1
|
| DOI |
10.1271/bbb.90615
|
| PII |
JST.JSTAGE/bbb/90615
|
| PMID |
20208360
|
| MeSH |
Amino Acid Sequence
Antineoplastic Agents / pharmacology*
Bacillus thuringiensis / metabolism*
Bacillus thuringiensis Toxins
Bacterial Proteins / pharmacology*
Dithiothreitol / chemistry
Endotoxins / pharmacology*
Female
HeLa Cells
Hemolysin Proteins / pharmacology*
Hep G2 Cells
Hepatocytes / drug effects*
Hepatocytes / pathology
Humans
Liver Neoplasms / pathology*
Molecular Sequence Data
Uterine Cervical Neoplasms / pathology*
|
| IF |
1.516
|
| 引用数 |
26
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
