| RRC ID |
68164
|
| 著者 |
Thein HSS, Hashimoto K, Kawashima N, Noda S, Okiji T.
|
| タイトル |
Evaluation of the anti-inflammatory effects of surface-reaction-type pre-reacted glass-ionomer filler containing root canal sealer in lipopolysaccharide-stimulated RAW264.7 macrophages.
|
| ジャーナル |
Dent Mater J
|
| Abstract |
A prototype surface-reaction-type pre-reacted glass-ionomer (S-PRG) filler containing root canal sealer (S-PRG sealer) exhibits bioactive potential by releasing multiple ions. This study explored the suppressive effects and modes of action of S-PRG sealer extracts on proinflammatory cytokine expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Expression of proinflammatory cytokines was evaluated by RT-qPCR and ELISA. Expression of phosphorylated nuclear factor-kappa B (p-NF-kB) p65 was evaluated by western blotting. S-PRG sealer extracts significantly downregulated mRNA expression levels of interleukin (IL)-1α, IL-6, and TNF-α in LPS-stimulated RAW264.7 cells; the extracts also reduced the levels of IL-6 protein and p-NF-kB. In order to verify that Zn2+ was responsible for downregulation of proinflammatory cytokine expression, N,N,N',N'-tetrakis(2-pyridylmethyl) ethylenediamine (TPEN) was used as a heavy metal chelator with strong affinity for Zn2+. These effects were mitigated by TPEN. The application of ZnCl2 reproduced the actions of S-PRG sealer extracts. These data suggest that S-PRG sealer has anti-inflammatory potential involving heavy metal ions such as Zn2+.
|
| 巻・号 |
41(1)
|
| ページ |
150-158
|
| 公開日 |
2022-2-1
|
| DOI |
10.4012/dmj.2021-139
|
| PMID |
34602587
|
| MeSH |
Dental Pulp Cavity
Glass Ionomer Cements*
Lipopolysaccharides* / pharmacology
Macrophages
|
| IF |
1.359
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
