| RRC ID |
68401
|
| 著者 |
Hemmi M, Ikeda Y, Shindo Y, Nakajima T, Nishiyama S, Oka K, Sato M, Hiruta Y, Citterio D, Suzuki K.
|
| タイトル |
Highly Sensitive Bioluminescent Probe for Thiol Detection in Living Cells.
|
| ジャーナル |
Chem Asian J
|
| Abstract |
The sensitive detection of thiols including glutathione and cysteine is desirable owing to their roles as indispensable biomolecules in maintaining intracellular biological redox homeostasis. Herein, we report the design and synthesis of SEluc-1 (sulfinate ester luciferin), a chemoselective probe exhibiting a ratiometric and turn-on response towards thiols selectively in fluorescence and bioluminescence, respectively. The probe, which was designed based on the "caged" luciferin strategy, displays excellent selectivity, high signal/noise ratio (>240 in the case of bioluminescence), and a biologically relevant limit of detection (LOD, 80 nm for cysteine), which are all desirable traits for a sensitive bioluminescent sensor. SEluc-1 was further applied to fluorescence imaging of thiol activity in living human cervical cancer HeLa cell cultures, and was successfully able to detect fluctuations in thiol concentrations induced by oxidative stress in a bioluminescent assay utilizing African green monkey fibroblast COS-7 cells and human breast adenocarcinoma MCF-7 cells.
|
| 巻・号 |
13(6)
|
| ページ |
648-655
|
| 公開日 |
2018-3-16
|
| DOI |
10.1002/asia.201701774
|
| PMID |
29359483
|
| MeSH |
Animals
COS Cells
Cell Survival
Chlorocebus aethiops
Firefly Luciferin*
Fluorescent Dyes*
HeLa Cells
Humans
Luminescent Measurements
MCF-7 Cells
Molecular Structure
Oxidative Stress
Sulfhydryl Compounds / analysis*
Sulfur Compounds*
Tumor Cells, Cultured
|
| IF |
4.056
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539)
MCF7(RCB1904) |
