RRC ID 68444
Author Horisawa-Takada Y, Kodera C, Takemoto K, Sakashita A, Horisawa K, Maeda R, Shimada R, Usuki S, Fujimura S, Tani N, Matsuura K, Akiyama T, Suzuki A, Niwa H, Tachibana M, Ohba T, Katabuchi H, Namekawa SH, Araki K, Ishiguro KI.
Title Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis.
Journal Nat Commun
Abstract During spermatogenesis, meiosis is accompanied by a robust alteration in gene expression and chromatin status. However, it remains elusive how the meiotic transcriptional program is established to ensure completion of meiotic prophase. Here, we identify a protein complex that consists of germ-cell-specific zinc-finger protein ZFP541 and its interactor KCTD19 as the key transcriptional regulators in mouse meiotic prophase progression. Our genetic study shows that ZFP541 and KCTD19 are co-expressed from pachytene onward and play an essential role in the completion of the meiotic prophase program in the testis. Furthermore, our ChIP-seq and transcriptome analyses identify that ZFP541 binds to and suppresses a broad range of genes whose function is associated with biological processes of transcriptional regulation and covalent chromatin modification. The present study demonstrates that a germ-cell specific complex that contains ZFP541 and KCTD19 promotes the progression of meiotic prophase towards completion in male mice, and triggers the reconstruction of the transcriptional network and chromatin organization leading to post-meiotic development.
Volume 12(1)
Pages 3184
Published 2021-6-1
DOI 10.1038/s41467-021-23378-4
PII 10.1038/s41467-021-23378-4
PMID 34075040
PMC PMC8169937
MeSH Animals Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Chromatin Immunoprecipitation Sequencing Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Disease Models, Animal Female Histone Deacetylase 1 / metabolism Histone Deacetylase 2 / metabolism Humans Infertility, Male / genetics Male Mice Mice, Knockout Nuclear Proteins / genetics Nuclear Proteins / metabolism* Oocytes / cytology Oocytes / metabolism Pachytene Stage / genetics* Potassium Channels, Voltage-Gated / genetics Potassium Channels, Voltage-Gated / metabolism* RNA-Seq Spermatids / cytology* Spermatids / metabolism Spermatogenesis / genetics* Transcription Factors / genetics Transcription Factors / metabolism* Transcription, Genetic
IF 12.121
Resource
DNA material pET28C-KCTD19 N (RDB19174) pET28C-KCTD19 C (RDB19175) pET19b-HFS2BP-Full (RDB19176) pET28C-4930432K21Rik-N (RDB19177)