論文 - 詳細
| RRC ID | 68444 |
|---|---|
| 著者 | Horisawa-Takada Y, Kodera C, Takemoto K, Sakashita A, Horisawa K, Maeda R, Shimada R, Usuki S, Fujimura S, Tani N, Matsuura K, Akiyama T, Suzuki A, Niwa H, Tachibana M, Ohba T, Katabuchi H, Namekawa SH, Araki K, Ishiguro KI. |
| タイトル | Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis. |
| ジャーナル | Nat Commun |
| Abstract |
During spermatogenesis, meiosis is accompanied by a robust alteration in gene expression and chromatin status. However, it remains elusive how the meiotic transcriptional program is established to ensure completion of meiotic prophase. Here, we identify a protein complex that consists of germ-cell-specific zinc-finger protein ZFP541 and its interactor KCTD19 as the key transcriptional regulators in mouse meiotic prophase progression. Our genetic study shows that ZFP541 and KCTD19 are co-expressed from pachytene onward and play an essential role in the completion of the meiotic prophase program in the testis. Furthermore, our ChIP-seq and transcriptome analyses identify that ZFP541 binds to and suppresses a broad range of genes whose function is associated with biological processes of transcriptional regulation and covalent chromatin modification. The present study demonstrates that a germ-cell specific complex that contains ZFP541 and KCTD19 promotes the progression of meiotic prophase towards completion in male mice, and triggers the reconstruction of the transcriptional network and chromatin organization leading to post-meiotic development. |
| 巻・号 | 12(1) |
| ページ | 3184 |
| 公開日 | 2021-6-1 |
| DOI | 10.1038/s41467-021-23378-4 |
| PII | 10.1038/s41467-021-23378-4 |
| PMID | 34075040 |
| PMC | PMC8169937 |
| MeSH | Animals Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Chromatin Immunoprecipitation Sequencing Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Disease Models, Animal Female Histone Deacetylase 1 / metabolism Histone Deacetylase 2 / metabolism Humans Infertility, Male / genetics Male Mice Mice, Knockout Nuclear Proteins / genetics Nuclear Proteins / metabolism* Oocytes / cytology Oocytes / metabolism Pachytene Stage / genetics* Potassium Channels, Voltage-Gated / genetics Potassium Channels, Voltage-Gated / metabolism* RNA-Seq Spermatids / cytology* Spermatids / metabolism Spermatogenesis / genetics* Transcription Factors / genetics Transcription Factors / metabolism* Transcription, Genetic |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pET28C-KCTD19 C (RDB19175) |