RRC ID 68491
著者 Ohno S, Noshiro M, Makihira S, Kawamoto T, Shen M, Yan W, Kawashima-Ohya Y, Fujimoto K, Tanne K, Kato Y.
タイトル RGD-CAP ((beta)ig-h3) enhances the spreading of chondrocytes and fibroblasts via integrin alpha(1)beta(1).
ジャーナル Biochim Biophys Acta
Abstract In previous studies, RGD-CAP (collagen-associated protein containing the RGD sequence) isolated from a collagen fiber-rich fraction of pig cartilage was found to be orthologous to human (beta)ig-h3, which is synthesized by lung adenocarcinoma cells in response to transforming growth factor-beta. In the present study, we examined the effect of recombinant chick RGD-CAP on the spreading of chondrocytes and fibroblasts using RGD-CAP-coated dishes. When rabbit articular chondrocytes, chick embryonic sternal chondrocytes, rabbit peritoneal fibroblasts or human MRC5 fibroblasts were seeded on plastic dishes coated with RGD-CAP, cell spreading was enhanced compared with that on control dishes (bovine serum albumin- or beta-galactosidase-coated dishes). The effect of RGD-CAP on the cell spreading required divalent cations (Mg(2+) or Mn(2+)), and was reduced by EDTA. Monoclonal antibodies (mAbs) to the human integrin alpha(1) or beta(1) subunit, but not to the alpha(2), alpha(3), alpha(5) or beta(2) subunits, suppressed the RGD-CAP-induced spreading of human MRC5 fibroblasts. In a parallel experiment, the mAb to the alpha(5) subunit, but not the mAb to the alpha(1) subunit, suppressed fibronectin-induced spreading of these cells. These findings suggest that RGD-CAP is a novel ligand for integrin alpha(1)beta(1) that dose not bind to the RGD motif. Accordingly, an RGD-CAP fragment, which carries a deletion in the C-terminal region containing the RGD motif, was still capable of stimulating cell spreading.
巻・号 1451(1)
ページ 196-205
公開日 1999-8-12
DOI 10.1016/s0167-4889(99)00093-2
PII S0167-4889(99)00093-2
PMID 10446401
MeSH Animals Antibodies, Monoclonal / pharmacology Cations, Divalent Cell Adhesion / drug effects Cell Culture Techniques / methods Cell Division / drug effects Cell Size / drug effects Chick Embryo Chondrocytes / drug effects* Cloning, Molecular Escherichia coli / metabolism Extracellular Matrix Proteins* Fibroblasts / drug effects* Fibronectins Humans Integrin alpha1beta1 Integrins / chemistry Integrins / immunology Integrins / metabolism* Neoplasm Proteins / genetics Neoplasm Proteins / immunology Neoplasm Proteins / pharmacology* Peptide Fragments / pharmacology Rabbits Transforming Growth Factor beta*
IF 3.411
リソース情報
ヒト・動物細胞 MRC-5