Reference - Detail
| RRC ID | 68516 |
|---|---|
| Author | Asano A, Ko JH, Morozumi T, Hamashima N, Watanabe T. |
| Title | Polymorphisms and the antiviral property of porcine Mx1 protein. |
| Journal | J Vet Med Sci |
| Abstract |
We determined the cDNA sequences of the type I interferon-inducible proteins, pig Mx1 from PK(15) and LLC-PK1 cells, and compared the antiviral activities of both Mx proteins, including Mx1 polymorphisms against vesicular stomatitis virus (VSV). Mx1 cDNA derived from PK(15) cells had an 11 bp-deletion in the 3' end of the coding region, and was estimated to encode 8 amino acid substitutions and a 23 amino acid extension compared to that from LLC-PK1 cells. VSV replication was inhibited in the 3T3 cells expressing Mx1 mRNA after the cDNA was transfected. However, the efficiency of this inhibition was not different between the cells expressing Mx1 mRNA from both PK and LLC. These results indicate that pig Mx1 protein confers resistance to VSV. |
| Volume | 64(12) |
| Pages | 1085-9 |
| Published | 2002-12-1 |
| DOI | 10.1292/jvms.64.1085 |
| PMID | 12520098 |
| MeSH | 3T3 Cells Amino Acid Sequence Animals Antiviral Agents / chemistry Antiviral Agents / genetics Antiviral Agents / metabolism Base Sequence Cell Line Cloning, Molecular DNA, Complementary / genetics GTP-Binding Proteins / chemistry GTP-Binding Proteins / genetics* GTP-Binding Proteins / metabolism* Gene Expression Mice Molecular Sequence Data Myxovirus Resistance Proteins Polymorphism, Genetic* RNA, Messenger / genetics RNA, Messenger / metabolism Swine / genetics* Transfection Vesicular stomatitis Indiana virus / growth & development Virus Replication |
| IF | 1.049 |
| Resource | |
| Human and Animal Cells | 3T3 |