論文 - 詳細
| RRC ID | 68517 |
|---|---|
| 著者 | Ishii TM, Takano M, Ohmori H. |
| タイトル | Determinants of activation kinetics in mammalian hyperpolarization-activated cation channels. |
| ジャーナル | J Physiol |
| Abstract |
1. The structural basis for the different activation kinetics of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels was investigated with the whole-cell patch clamp technique by using HCN1, HCN4, chimeric channels and mutants in a mammalian expression system (COS-7). 2. The activation time constant of HCN4 was about 40-fold longer than that of HCN1 when compared at -100 mV. 3. In chimeras between HCN1 and HCN4, the region of the S1 transmembrane domain and the exoplasmic S1-S2 linker markedly affected the activation kinetics. The cytoplasmic region between S6 and the cyclic nucleotide-binding domain (CNBD) also significantly affected the activation kinetics. 4. The S1 domain and S1-S2 linker of HCN1 differ from those of HCN4 at eight amino acid residues, and each single point mutation of them changed the activation kinetics less than 2-fold. However, the effects of those mutations were additive and the substitution of the whole S1 and S1-S2 region of HCN1 by that of HCN4 resulted in a 10- to 20-fold slowing. 5. The results indicate that S1 and S1-S2, and S6-CNBD are the crucial components for the activation gating of HCN channels. |
| 巻・号 | 537(Pt 1) |
| ページ | 93-100 |
| 公開日 | 2001-11-15 |
| DOI | 10.1111/j.1469-7793.2001.0093k.x |
| PII | PHY_12629 |
| PMID | 11711564 |
| PMC | PMC2278938 |
| MeSH | Animals COS Cells Cations / metabolism* Chimera Cyclic Nucleotide-Gated Cation Channels Electrophysiology Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Ion Channels / genetics Ion Channels / physiology* Kinetics Mice Muscle Proteins* Nerve Tissue Proteins* Point Mutation Potassium Channels Protein Structure, Tertiary / physiology Rabbits Time Factors |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |