RRC ID 68523
著者 Udono T, Takahashi K, Yasumoto K, Yoshizawa M, Takeda K, Abe T, Tamai M, Shibahara S.
タイトル Expression of tyrosinase-related protein 2/DOPAchrome tautomerase in the retinoblastoma.
ジャーナル Exp Eye Res
Abstract Tyrosinase-related protein 2 (TRP-2), also known as DOPAchrome tautomerase, is an enzyme in melanin biosynthesis and may play an important role in detoxification of a metabolite derived from DOPA. TRP-2 is expressed in melanocytes of neural crest origin and retinal pigment epithelium (RPE), derived from the optic cup. TRP-2 has been established as an early differentiation marker for melanoblasts and RPE. It is therefore of significance to study the regulation of TRP-2/DOPAchrome tautomerase expression. Here we show that TRP-2 mRNA is expressed in Y79 human retinoblastoma cell line, derived from a primitive multipotential retinal cell. Retinoblastoma is the common primary intraocular tumor of childhood. Basal expression levels in Y79 retinoblastoma cells of TRP-2 mRNA and protein are comparable to those in melanoma cells, whereas mRNA for tyrosinase, the rate-limiting enzyme in melanogenesis, is undetectable in retinoblastoma cells. Transient transfection assays showed that the TRP-2 gene promoter efficiently directs the reporter gene expression in retinoblastoma cells as it does in melanoma cells. Moreover, the expression of TRP-2 mRNA was induced by retinoic acid in retinoblastoma cells but not noticeably affected by forskolin, a cAMP-elevating reagent, whereas in melanoma cells its expression was induced by forskolin but not by retinoic acid. These results suggest a difference in the regulation of TRP-2 expression between retinoblastoma and melanoma cells. Moreover, TRP-2 mRNA is expressed in the excised retinoblastoma specimens, as assessed by RT-PCR. The present study shows unexpected features of TRP-2 and may enhance our understanding of the pathophysiology of retinoblastoma.
巻・号 72(3)
ページ 225-34
公開日 2001-3-1
DOI 10.1006/exer.2000.0948
PII S0014483500909485
PMID 11180971
MeSH Colforsin / pharmacology Gene Expression Genes, Reporter Humans Melanoma / enzymology Monophenol Monooxygenase / genetics Monophenol Monooxygenase / metabolism* RNA, Messenger / metabolism Retinal Neoplasms / enzymology* Retinoblastoma / enzymology* Reverse Transcriptase Polymerase Chain Reaction Tretinoin / physiology Tumor Cells, Cultured
IF 3.011
リソース情報
ヒト・動物細胞 HMV-II(RCB0777) Y79(RCB0427)