Reference - Detail
| RRC ID | 68524 |
|---|---|
| Author | de Pooter RF, Cho SK, Zúñiga-Pflücker JC. |
| Title | In vitro generation of lymphocytes from embryonic stem cells. |
| Journal | Methods Mol Biol |
| Abstract |
Lymphocytes arise during ontogeny via a series of increasingly restricted intermediates. Initially, the mesoderm gives rise to hemangioblasts, which can differentiate into endothelial precursors, or hematopoietic stem cells (HSCs). HSCs can either self-renew or differentiate into lineage-restricted progenitors and, ultimately, to mature effector cells. This complex process is only beginning to be understood, and the ability to generate lymphocytes from embryonic stem (ES) cells in vitro will facilitate further study by providing a model system in which the effects of genetic and environmental manipulations of ES-cell-derived progenitors can be examined. In this protocol, we describe procedures for generating either B- and NK- or T-lymphocytes from mouse ES cells in vitro. |
| Volume | 290 |
| Pages | 135-47 |
| Published | 2005-1-1 |
| DOI | 10.1385/1-59259-838-2:135 |
| PII | 1-59259-838-2:135 |
| PMID | 15361660 |
| MeSH | Animals Coculture Techniques Embryo, Mammalian / cytology* Lymphocytes / classification Lymphocytes / cytology* Mice Mice, Inbred BALB C Mice, Inbred C57BL Stem Cells / cytology* |
| Altmetric score |
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | OP9(RCB1124) |