論文 - 詳細
| RRC ID | 68542 |
|---|---|
| 著者 | Rahman T, Taylor CW. |
| タイトル | Nuclear patch-clamp recording from inositol 1,4,5-trisphosphate receptors. |
| ジャーナル | Methods Cell Biol |
| Abstract |
Inositol 1,4,5-trisphosphate receptors (IP(3)R) are ubiquitous intracellular Ca(2+) channels. They are regulated by IP(3) and Ca(2+) and can thereby both initiate local Ca(2+) release events and regeneratively propagate Ca(2+) signals evoked by receptors that stimulate IP(3) formation. Local signaling by small numbers of IP(3)R underpins the utility of IP(3)-evoked Ca(2+) signals as a ubiquitous signaling pathway. The physiological impact of Ca(2+) release by very small numbers of IP(3)R underscores the necessity to understand the behavior of IP(3)R at the single-channel level. In addition, and in common with analyses of every other ion channel, single-channel analyses have the potential to define the steps linking IP(3) binding to channel opening. Patch-clamp recording, by resolving the openings and closings of single channels with exquisite temporal resolution, is the most powerful technique for analysis of single-channel events. It has contributed enormously to the understanding of gating and desensitization/inactivation of numerous ion channels. However, most IP(3)R reside within intracellular membranes, where they are inaccessible to conventional patch-clamp recording methods. Here, we describe the application of nuclear patch-clamp methods to single-channel analyses of native and recombinant IP(3)R. |
| 巻・号 | 99 |
| ページ | 199-224 |
| 公開日 | 2010-1-1 |
| DOI | 10.1016/B978-0-12-374841-6.00008-6 |
| PII | B978-0-12-374841-6.00008-6 |
| PMID | 21035688 |
| MeSH | Animals Cations Cell Line Cell Nucleus / metabolism* Chickens Gene Knockout Techniques Inositol 1,4,5-Trisphosphate Receptors / metabolism* Ion Channel Gating Patch-Clamp Techniques / methods* |
| IF | 1.441 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | DT40(RCB1464) IP3R^(-) DT40(RCB1467) |