RRC ID 68585
著者 Ota A, Tajima M, Mori K, Sugiyama E, Sato VH, Sato H.
タイトル The selective cytotoxicity of silver thiosulfate, a silver complex, on MCF-7 breast cancer cells through ROS-induced cell death.
ジャーナル Pharmacol Rep
Abstract BACKGROUND:Silver is a transition metal that is known to be less toxic than platinum. However, only few studies have reported the anticancer effects of some silver complexes and their possibility as an alternative to platinum complex. This study investigated the anticancer effects of the silver thiosulfate complex (STS), [Ag(S2O3)2]3-, consisting of silver and sodium thiosulfate.
METHODS:In vitro cytotoxic activity of STS was investigated comparatively in human cancer cell lines (K562 and MCF-7) and normal human cells (mesenchymal stem cells and mammary epithelial cells). For its anticancer effects, cell cycle, mode of cell death, morphological changes, and accumulation of intracellular ROS and GSH were evaluated in MCF-7 to provide mechanistic insights.
RESULTS:STS showed a concentration-dependent cytotoxicity in MCF-7 cell, which was abolished by pretreatment with N-acetylcysteine, suggesting ROS accumulation by STS. Moreover, STS caused cell cycle arrest at the G1 phase, decrease in the GSH levels, and morphological changes in MCF-7. Direct measurement of ROS demonstrated the elevation of intracellular ROS accumulation in cancer cells treated with STS; however, neither cytotoxicity nor ROS accumulation was observed in normal human cells.
CONCLUSION:The results obtained here are the first evidence to show that STS exhibited an anticancer activity through ROS-induced mechanisms, and that its cytotoxicity is highly selective to cancer cells. The results of the present study warrant further investigation on the detailed mechanism of STS actions, as well as its in vivo effectiveness and safety for clinical application.
巻・号 73(3)
ページ 847-857
公開日 2021-6-1
DOI 10.1007/s43440-021-00260-0
PII 10.1007/s43440-021-00260-0
PMID 33864630
PMC PMC8180477
MeSH Antineoplastic Agents / pharmacology* Apoptosis / drug effects Breast Neoplasms / drug therapy* Breast Neoplasms / metabolism* Cell Cycle Checkpoints / drug effects Cell Death / drug effects* Cell Line, Tumor Female G1 Phase / drug effects Humans K562 Cells MCF-7 Cells Reactive Oxygen Species / metabolism* Silver / pharmacology* Thiosulfates / pharmacology*
IF 2.761
リソース情報
ヒト・動物細胞 K562