論文 - 詳細
| RRC ID | 68587 |
|---|---|
| 著者 | Fujii D, Takase K, Takagi A, Kamino K, Hirano Y. |
| タイトル | Design of RGDS Peptide-Immobilized Self-Assembling β-Strand Peptide from Barnacle Protein. |
| ジャーナル | Int J Mol Sci |
| Abstract |
We designed three types of RGD-containing barnacle adhesive proteins using self-assembling peptides. In the present study, three types of RGD-containing peptides were synthesized by solid-phase peptide synthesis, and the secondary structures of these peptides were analyzed by CD and FT-IR spectroscopy. The mechanical properties of peptide hydrogels were characterized by a rheometer. We discuss the correlation between the peptide conformation, and cell attachment and cell spreading activity from the viewpoint of developing effective tissue engineering scaffolds. We created a peptide-coated cell culture substrate by coating peptides on a polystyrene plate. They significantly facilitated cell adhesion and spreading compared to a non-coated substrate. When the RGDS sequence was modified at N- or C-terminal of R-Y, it was found that the self-assembling ability was dependent on the strongly affects hydrogel formation and cell adhesion caused by its secondary structure. |
| 巻・号 | 22(3) |
| 公開日 | 2021-1-27 |
| DOI | 10.3390/ijms22031240 |
| PII | ijms22031240 |
| PMID | 33513895 |
| PMC | PMC7866236 |
| MeSH | Animals Immobilized Proteins / chemistry* Immobilized Proteins / genetics Immobilized Proteins / ultrastructure Peptides / chemistry* Peptides / genetics Protein Conformation, beta-Strand / genetics* Proteins / chemistry* Proteins / ultrastructure Thoracica / chemistry Thoracica / genetics |
| IF | 4.556 |
| リソース情報 | |
| ヒト・動物細胞 | L929(RCB1451) |