RRC ID 68600
Author Kato S, Arasaki K, Tokutomi N, Imai Y, Inoshita T, Hattori N, Sasaki T, Sato M, Wakana Y, Inoue H, Tagaya M.
Title Syntaxin 17, an ancient SNARE paralog, plays different and conserved roles in different organisms.
Journal J Cell Sci
Abstract Mammalian syntaxin 17 (Stx17) has several functions, other than membrane fusion, including mitochondrial division, autophagosome formation and lipid droplet expansion. Different from conventional syntaxins, Stx17 has a long C-terminal hydrophobic region with a hairpin-like structure flanked by a basic amino acid-enriched C-terminal tail. Although Stx17 is one of the six ancient SNAREs and present in diverse eukaryotic organisms, it has been lost in multiple lineages during evolution. In the present study, we compared the localization and function of fly and nematode Stx17s expressed in HeLa cells with those of human Stx17. We found that fly Stx17 predominantly localizes to the cytosol and mediates autophagy, but not mitochondrial division. Nematode Stx17, on the other hand, is predominantly present in mitochondria and facilitates mitochondrial division, but is irrelevant to autophagy. These differences are likely due to different structures in the C-terminal tail. Non-participation of fly Stx17 and nematode Stx17 in mitochondrial division and autophagy, respectively, was demonstrated in individual organisms. Our results provide an insight into the evolution of Stx17 in metazoa.
Volume 134(22)
Published 2021-11-15
DOI 10.1242/jcs.258699
PII 272626
PMID 34704591
MeSH Animals Autophagy HeLa Cells Humans Membrane Fusion* Qa-SNARE Proteins / genetics SNARE Proteins*
IF 4.573
Human and Animal Cells HeLa(RCB0007) HuH-7(RCB1942) THP-1(RCB1189) S2 (Drosophila)(RCB1153)
Drosophila DGRC#140948