| RRC ID |
68617
|
| Author |
Kuribayashi-Shigetomi K, Onoe H, Takeuchi S.
|
| Title |
Cell origami: self-folding of three-dimensional cell-laden microstructures driven by cell traction force.
|
| Journal |
PLoS One
|
| Abstract |
This paper describes a method of generating three-dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF). We harness the CTF as a biological driving force to fold the microstructures. Cells stretch and adhere across multiple microplates. Upon detaching the microplates from a substrate, CTF causes the plates to lift and fold according to a prescribed pattern. This self-folding technique using cells is highly biocompatible and does not involve special material requirements for the microplates and hinges to induce folding. We successfully produced various 3D cell-laden microstructures by just changing the geometry of the patterned 2D plates. We also achieved mass-production of the 3D cell-laden microstructures without causing damage to the cells. We believe that our methods will be useful for biotechnology applications that require analysis of cells in 3D configurations and for self-assembly of cell-based micro-medical devices.
|
| Volume |
7(12)
|
| Pages |
e51085
|
| Published |
2012-1-1
|
| DOI |
10.1371/journal.pone.0051085
|
| PII |
PONE-D-12-15395
|
| PMID |
23251426
|
| PMC |
PMC3521028
|
| MeSH |
Animals
Cell Shape*
Mice
NIH 3T3 Cells
|
| IF |
2.74
|
| Resource |
| Human and Animal Cells |
NIH/3T3(RCB2767) |
