RRC ID 68621
著者 Ostalé CM, Esteban N, López-Varea A, de Celis JF.
タイトル Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.
ジャーナル G3 (Bethesda)
Abstract Protein kinases and phosphatases constitute a large family of conserved enzymes that control a variety of biological processes by regulating the phosphorylation state of target proteins. They play fundamental regulatory roles during cell cycle progression and signaling, among other key aspects of multicellular development. The complement of protein kinases and phosphatases includes approximately 326 members in Drosophila, and they have been the subject of several functional screens searching for novel components of signaling pathways and regulators of cell division and survival. These approaches have been carried out mostly in cell cultures using RNA interference to evaluate the contribution of each protein in different functional assays, and have contributed significantly to assign specific roles to the corresponding genes. In this work we describe the results of an evaluation of the Drosophila complement of kinases and phosphatases using the wing as a system to identify their functional requirements in vivo. We also describe the results of several modifying screens aiming to identify among the set of protein kinases and phosphatases additional components or regulators of the activities of the Epidermal Growth Factor and Insulin receptors signaling pathways.
巻・号 11(12)
公開日 2021-12-8
DOI 10.1093/g3journal/jkab348
PII 6380433
PMID 34599799
PMC PMC8664455
MeSH Animals Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Phosphoric Monoester Hydrolases Protein Kinases / genetics RNA Interference Wings, Animal / metabolism
IF 2.781
リソース情報
ショウジョウバエ 10539R-3 4012R-2 5092R-2 9738R-1 1891R-3 7904R-2 10079R-2 7525R-2