| RRC ID |
6863
|
| Author |
Wang S, Kawashima N, Sakamoto K, Katsube K, Umezawa A, Suda H.
|
| Title |
Osteogenic differentiation of mouse mesenchymal progenitor cell, Kusa-A1 is promoted by mammalian transcriptional repressor Rbpj.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Pluripotent mesenchymal stem cells possess the ability to differentiate into many cell types, but the precise mechanisms of differentiation are still unclear. Here, we provide evidence that Rbpj (recombination signal-binding protein for immunoglobulin kappa j region) protein, the primary nuclear mediator of Notch, is involved in osteogenesis. Overexpression of Rbpj promoted osteogenic differentiation of mouse Kusa-A1 cells in vitro and in vivo. Transient transfection of an Rbpj expression vector into Kusa-A1 cells upregulated promoter activities of Runx2 and Ose2. Enhanced osteogenic potentials including high alkaline phosphatase activity, rapid calcium deposition, and increased calcified nodule formation, were observed in established stable Rbpj-overexpressing Kusa-A1 (Kusa-A1/Rbpj) cell line. In vivo mineralization by Kusa-A1/Rbpj was promoted compared to that by Kusa-A1 host cells. Histological findings revealed that expression of Rbpj was primarily observed in osteoblasts. These results suggest that Rbpj may play essential roles in osteoblast differentiation.
|
| Volume |
400(1)
|
| Pages |
39-45
|
| Published |
2010-9-10
|
| DOI |
10.1016/j.bbrc.2010.07.133
|
| PII |
S0006-291X(10)01466-X
|
| PMID |
20691157
|
| MeSH |
Animals
Calcification, Physiologic / genetics
Cell Line
Core Binding Factor Alpha 1 Subunit / genetics
Gene Expression Regulation, Developmental*
Immunoglobulin J Recombination Signal Sequence-Binding Protein / genetics
Immunoglobulin J Recombination Signal Sequence-Binding Protein / metabolism*
Mesenchymal Stem Cells / cytology*
Mice
Osteogenesis / genetics*
Pluripotent Stem Cells / cytology*
Pluripotent Stem Cells / metabolism
Promoter Regions, Genetic
Receptors, Notch / genetics
|
| IF |
2.985
|
| Times Cited |
5
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pEF5/FRT/V5/Rbpj (RDB08126)
pTag/HA/Rpbj (RDB08127)
RBP-J (RDB01801) |
