Reference - Detail
|Author||Kawatani M, Takayama H, Muroi M, Kimura S, Maekawa T, Osada H.|
|Title||Identification of a small-molecule inhibitor of DNA topoisomerase II by proteomic profiling.|
BNS-22, a chemically synthesized derivative of the natural plant product GUT-70, has antiproliferative activity against human cancer cells, the mechanism of which is unknown. Here, we identify a target of BNS-22 by proteomic profiling analysis, which suggests that BNS-22 belongs to the same cluster as ICRF-193, a DNA topoisomerase II (TOP2) catalytic inhibitor. BNS-22 inhibits kinetoplast DNA decatenation that is mediated by human TOP2α and TOP2β in vitro at an IC(50) of 2.8 and 0.42 μM, respectively. BNS-22 does not affect DNA damage and antagonizes TOP2 poison-mediated DNA damage. Like ICRF-193, BNS-22 induces mitotic abnormalities, characterized by impairments in chromosome alignment and segregation, thereby causing polyploidy in HeLa cells. These results indicate that BNS-22 targets TOP2 and acts as its catalytic inhibitor.
|MeSH||Cluster Analysis Coumarins / chemistry* Coumarins / toxicity DNA Topoisomerases, Type II / chemistry* DNA Topoisomerases, Type II / metabolism Electrophoresis, Gel, Two-Dimensional HeLa Cells Humans Proteome / metabolism* Proteomics / methods Quinolines / chemistry* Quinolines / toxicity Spindle Apparatus / drug effects Structure-Activity Relationship Topoisomerase II Inhibitors / chemistry* Topoisomerase II Inhibitors / toxicity|
|Human and Animal Cells||HeLa(RCB0007)|