論文 - 詳細
| RRC ID | 68649 |
|---|---|
| 著者 | Nojima T, Haniuda K, Moutai T, Matsudaira M, Mizokawa S, Shiratori I, Azuma T, Kitamura D. |
| タイトル | In-vitro derived germinal centre B cells differentially generate memory B or plasma cells in vivo. |
| ジャーナル | Nat Commun |
| Abstract |
In response to T cell-dependent antigens, B cells proliferate extensively to form germinal centres (GC), and then differentiate into memory B (B(mem)) cells or long-lived plasma cells (LLPCs) by largely unknown mechanisms. Here we show a new culture system in which mouse naïve B cells undergo massive expansion and isotype switching, and generate GC-phenotype B (iGB) cells. The iGB cells expressing IgG1 or IgM/D, but not IgE, differentiate into B(mem) cells in vivo after adoptive transfer and can elicit rapid immune responses with the help of cognate T cells. Secondary culture with IL-21 maintains the proliferation of the iGB cells, while shifting their in vivo developmental fate from B(mem) cells to LLPCs, an outcome that can be reversed by withdrawal of IL-21 in tertiary cultures. Thus, this system enables in vitro manipulation of B-cell fate, into either B(mem) cells or LLPCs, and will facilitate dissection of GC-B cell differentiation programs. |
| 巻・号 | 2 |
| ページ | 465 |
| 公開日 | 2011-9-6 |
| DOI | 10.1038/ncomms1475 |
| PII | ncomms1475 |
| PMID | 21897376 |
| MeSH | 3T3 Cells Animals Antigens / immunology B-Lymphocytes / cytology B-Lymphocytes / immunology* Cell Proliferation Flow Cytometry Immunologic Memory* In Vitro Techniques Interleukin-4 / physiology Interleukins / physiology Mice Mice, Inbred BALB C Mice, Inbred C57BL Plasma Cells / cytology Plasma Cells / immunology* |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BALB/3T3 clone A31(RCB0005) |