RRC ID 68654
著者 Xu L, Xiong F, Bai Y, Xiao J, Zhang Y, Chen J, Li Q.
タイトル Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome.
ジャーナル Reprod Biol Endocrinol
Abstract BACKGROUND:Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disease in women at childbearing age. Several circular RNAs (circRNAs) have been demonstrated to be involved in PCOS. In this study, we aimed to explore the function and mechanism of circ_0043532 in PCOS.
METHODS:Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the expression of circ_0043532, miR-182 and serum/glucocorticoid regulated kinase family member 3 (SGK3). Cell proliferation was assessed by 5-ethynyl-2'-deoxyuridine (EdU) assay and 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Flow cytometry analysis was employed to evaluate cell cycle and cell apoptosis. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were conducted to verify the association between miR-182 and SGK3. Western blot assay was carried out to determine the protein level of SGK3.
RESULTS:Circ_0043532 was markedly elevated in PCOS granulosa cells (GCs) and KGN cells. Silencing of circ_0043532 suppressed cell proliferation and cell cycle process and promoted cell apoptosis in PCOS GCs and KGN cells. For mechanistic analysis, circ_0043532 was identified as a sponge of miR-182 and SGK3 was confirmed to be a target gene of miR-182. Inhibition of miR-182 rescued the impacts of circ_0043532 interference on PCOS GCs and KGN cell progression. Moreover, miR-182 overexpression suppressed cell proliferation and cell cycle process and promoted cell apoptosis in PCOS GCs and KGN cells by targeting SGK3.
CONCLUSION:Deficiency of circ_0043532 suppressed cell proliferation and induced cell cycle arrest and cell apoptosis in PCOS by modulation of miR-182/SGK3 axis.
巻・号 19(1)
ページ 167
公開日 2021-11-5
DOI 10.1186/s12958-021-00839-5
PII 10.1186/s12958-021-00839-5
PMID 34740363
PMC PMC8569971
MeSH Adult Cell Movement / physiology Cell Proliferation / physiology* Female Granulosa Cells / metabolism* Granulosa Cells / pathology Humans MicroRNAs / biosynthesis* MicroRNAs / genetics Polycystic Ovary Syndrome / genetics Polycystic Ovary Syndrome / metabolism* Polycystic Ovary Syndrome / pathology Protein Serine-Threonine Kinases / biosynthesis* Protein Serine-Threonine Kinases / genetics RNA, Circular / biosynthesis* RNA, Circular / genetics Young Adult
IF 3.235
リソース情報
ヒト・動物細胞 KGN(RCB1154)