Reference - Detail
RRC ID | 68657 |
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Author | Zheng C, Liu S, Qin Z, Zhang X, Song Y. |
Title | LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis. |
Journal | J Ovarian Res |
Abstract |
BACKGROUND:miR-146b-5p has been reported to participate in premature ovarian failure (POF) in mice. However, its role in POF patients is unclear. We predicted that miR-146b-5p might interact with lncRNA DLEU1, a crucial player in ovarian cancer. We then explored the interaction between DLEU1 and miR-146b-5p. METHODS:Expression of DLEU1 and miR-146b-5p in POF and control ovary tissues was determined by RT-qPCR. The subcellular location of DLEU1 in human KGN cells was analyzed using subcellular fractionation assays. The direct interaction between DLEU1 and miR-146b-5p was analyzed using RNA pull-down assays. The role of DLEU1 in miR-146a expression was analyzed using overexpression assay. Cell proliferation was analyzed using cell apoptosis assay. RESULTS:Increased DLEU1 expression and decreased miR-146b-5p expression were observed in POF. DLEU1 directly interacted with MiR-146b-5p and was expressed in both nuclear and cytoplasm samples of KGN cells. In KGN cells, DLEU1 and miR-146b-5p failed to regulate the expression of each other. However, DLEU1 promoted cell apoptosis and reduced the inhibitory effects of miR-146b-5p on cell apoptosis. CONCLUSIONS:DLEU1 is overexpressed in POF and sponges miR-146b-5p to increase KGN cell apoptosis. |
Volume | 14(1) |
Pages | 151 |
Published | 2021-11-5 |
DOI | 10.1186/s13048-021-00905-x |
PII | 10.1186/s13048-021-00905-x |
PMID | 34740384 |
PMC | PMC8569989 |
MeSH | Adult Anti-Mullerian Hormone / blood Apoptosis / genetics* Case-Control Studies Cell Proliferation / genetics* Estradiol / blood Female Follicle Stimulating Hormone / blood Granulosa Cells* Humans Luteinizing Hormone / blood MicroRNAs / genetics* Primary Ovarian Insufficiency / blood Primary Ovarian Insufficiency / genetics* RNA, Long Noncoding / genetics* |
IF | 2.469 |
Resource | |
Human and Animal Cells | KGN(RCB1154) |