RRC ID 68672
著者 Shi L, Kong R, Li Z, Zhao H, Ma R, Bai G, Li J, Li Z.
タイトル Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation.
ジャーナル Biol Open
Abstract Adult stem cells are critical for the maintenance of tissue homeostasis. However, how the proliferation and differentiation of intestinal stem cells (ISCs) are regulated remains not fully understood. Here, we find a mutant, stum 9-3, affecting the proliferation and differentiation of Drosophila adult ISCs in a forward genetic screen for factors regulating the proliferation and differentiation ISCs. stum 9-3 acts through the conserved Notch signaling pathway, upstream of the S2 cleavage of the Notch receptor. Interestingly, the phenotype of stum 9-3 mutant is not caused by disruption of stumble (stum), where the p-element is inserted. Detailed mapping, rescue experiments and mutant characterization show that stum 9-3 is a new allele of O-fucosyltransferase 1 (O-fut1). Our results indicate that unexpected mutants with interesting phenotype could be recovered in forward genetic screens using known p-element insertion stocks.
巻・号 10(11)
公開日 2021-11-15
DOI 10.1242/bio.058910
PII 272697
PMID 34731235
PMC PMC8576262
MeSH Alleles* Animals Drosophila / genetics* Drosophila Proteins / physiology* Fucosyltransferases / physiology* Intestines / cytology Stem Cells / metabolism*
IF 2.029
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