RRC ID 68723
Author Wang X, Ito A, Li X, Sogo Y, Hirose M, Oyane A, Tsurushima H.
Title DNA-lipid-apatite composite layers enhance gene expression of mesenchymal stem cells.
Journal Mater Sci Eng C Mater Biol Appl
Abstract DNA-apatite composite layer (D-Ap layer) and DNA-lipid-apatite composite layer (DLp-Ap layer) were prepared on ceramic hydroxyapatite disk and scaffold using supersaturated calcium phosphate solutions supplemented with 0.5-5 μg/mL plasmid and 0-10 μL/mL lipid transfection reagent FuGENE®. Both in vitro and in vivo studies were carried out using mesenchymal stem cells (MSCs) and two kinds of gene (luciferase and bone morphogenetic protein (BMP)-2) for demonstrating potential application of the gene transfer system using the D-Ap and DLp-Ap layers in bone tissue engineering. In the in vitro study using luciferase gene, the DLp-Ap layers showed 1-2 orders of magnitudes higher gene transfer efficiency to MSCs than the D-Ap layer. In the in vivo study using BMP-2 gene, DLp-Ap layer slightly increased BMP-2 protein concentration than D-Ap layer, thereby enhancing their osteogenic differentiation than D-Ap layer. The present gene transfer system using the DLp-Ap layers, with the advantages of good biocompatibility, bone-bonding ability, and efficacy in in vitro and in vivo gene transfer to MSCs, would be useful in bone tissue engineering.
Volume 33(1)
Pages 512-8
Published 2013-1-1
DOI 10.1016/j.msec.2012.09.023
PII S0928-4931(12)00455-9
PMID 25428103
MeSH Alkaline Phosphatase / metabolism Animals Apatites / pharmacology* Bone Morphogenetic Protein 2 / genetics Bone Morphogenetic Protein 2 / metabolism DNA / metabolism* Durapatite / pharmacology Gene Expression Regulation / drug effects* Lipids / pharmacology* Luciferases / genetics Mesenchymal Stem Cells / drug effects Mesenchymal Stem Cells / metabolism* Mice NIH 3T3 Cells Osteocalcin / metabolism Rats, Inbred F344 Skin / metabolism Tissue Scaffolds / chemistry Transfection X-Ray Diffraction
IF 5.88
Human and Animal Cells NIH3T3-3-4(RCB1862)