RRC ID |
68723
|
著者 |
Wang X, Ito A, Li X, Sogo Y, Hirose M, Oyane A, Tsurushima H.
|
タイトル |
DNA-lipid-apatite composite layers enhance gene expression of mesenchymal stem cells.
|
ジャーナル |
Mater Sci Eng C Mater Biol Appl
|
Abstract |
DNA-apatite composite layer (D-Ap layer) and DNA-lipid-apatite composite layer (DLp-Ap layer) were prepared on ceramic hydroxyapatite disk and scaffold using supersaturated calcium phosphate solutions supplemented with 0.5-5 μg/mL plasmid and 0-10 μL/mL lipid transfection reagent FuGENE®. Both in vitro and in vivo studies were carried out using mesenchymal stem cells (MSCs) and two kinds of gene (luciferase and bone morphogenetic protein (BMP)-2) for demonstrating potential application of the gene transfer system using the D-Ap and DLp-Ap layers in bone tissue engineering. In the in vitro study using luciferase gene, the DLp-Ap layers showed 1-2 orders of magnitudes higher gene transfer efficiency to MSCs than the D-Ap layer. In the in vivo study using BMP-2 gene, DLp-Ap layer slightly increased BMP-2 protein concentration than D-Ap layer, thereby enhancing their osteogenic differentiation than D-Ap layer. The present gene transfer system using the DLp-Ap layers, with the advantages of good biocompatibility, bone-bonding ability, and efficacy in in vitro and in vivo gene transfer to MSCs, would be useful in bone tissue engineering.
|
巻・号 |
33(1)
|
ページ |
512-8
|
公開日 |
2013-1-1
|
DOI |
10.1016/j.msec.2012.09.023
|
PII |
S0928-4931(12)00455-9
|
PMID |
25428103
|
MeSH |
Alkaline Phosphatase / metabolism
Animals
Apatites / pharmacology*
Bone Morphogenetic Protein 2 / genetics
Bone Morphogenetic Protein 2 / metabolism
DNA / metabolism*
Durapatite / pharmacology
Gene Expression Regulation / drug effects*
Lipids / pharmacology*
Luciferases / genetics
Mesenchymal Stem Cells / drug effects
Mesenchymal Stem Cells / metabolism*
Mice
NIH 3T3 Cells
Osteocalcin / metabolism
Rats, Inbred F344
Skin / metabolism
Tissue Scaffolds / chemistry
Transfection
X-Ray Diffraction
|
IF |
5.88
|
リソース情報 |
ヒト・動物細胞 |
NIH3T3-3-4(RCB1862) |