論文 - 詳細
| RRC ID | 68823 |
|---|---|
| 著者 | Miyagawa-Yamaguchi A, Kotani N, Honke K. |
| タイトル | Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains. |
| ジャーナル | PLoS One |
| Abstract |
Lipid rafts that are enriched in glycosylphosphatidylinositol (GPI)-anchored proteins serve as a platform for important biological events. To elucidate the molecular mechanisms of these events, identification of co-clustering molecules in individual raft domains is required. Here we describe an approach to this issue using the recently developed method termed enzyme-mediated activation of radical source (EMARS), by which molecules in the vicinity within 300 nm from horseradish peroxidase (HRP) set on the probed molecule are labeled. GPI-anchored HRP fusion proteins (HRP-GPIs), in which the GPI attachment signals derived from human decay accelerating factor and Thy-1 were separately connected to the C-terminus of HRP, were expressed in HeLa S3 cells, and the EMARS reaction was catalyzed by these expressed HRP-GPIs under a living condition. As a result, these different HRP-GPIs had differences in glycosylation and localization and formed distinct clusters. This novel approach distinguished molecular clusters associated with individual GPI-anchored proteins, suggesting that it can identify co-clustering molecules in individual raft domains. |
| 巻・号 | 9(3) |
| ページ | e93054 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0093054 |
| PII | PONE-D-13-28761 |
| PMID | 24671047 |
| PMC | PMC3966864 |
| MeSH | CD55 Antigens / metabolism Free Radicals / metabolism GPI-Linked Proteins / genetics GPI-Linked Proteins / metabolism* Glycosylation HeLa Cells Horseradish Peroxidase / genetics Horseradish Peroxidase / metabolism* Humans Membrane Microdomains / metabolism* Protein Transport Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Thy-1 Antigens / metabolism |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |