論文 - 詳細
| RRC ID | 68880 |
|---|---|
| 著者 | Chia JY, Miki T, Mihara H, Tsutsumi H. |
| タイトル | Biofunctional supramolecular hydrogels fabricated from a short self-assembling peptide modified with bioactive sequences for the 3D culture of breast cancer MCF-7 cells. |
| ジャーナル | Bioorg Med Chem |
| Abstract |
Self-assembling peptides are a type of molecule with promise as scaffold materials for cancer cell engineering. We have reported a short self-assembling peptide, (FFiK)2, that had a symmetric structure connected via a urea bond. In this study, we functionalized (FFiK)2 by conjugation with various bioactive sequences for the 3D culture of cancer cells. Four sequences, RGDS and PHSRN derived from fibronectin and AG73 and C16 derived from laminin, were selected as bioactive sequences to promote cell adhesion, proliferation or migration. (FFiK)2, and its derivatives could co-assemble into supramolecular nanofibers displaying bioactive sequences and form hydrogels. MCF-7 cells were encapsulated in functionalized peptide hydrogels without significant cytotoxicity. Encapsulated MCF-7 cells proliferated under 3D culture conditions. MCF-7 cells proliferated with spheroid formation in hydrogels that displayed RGDS or PHSRN sequences, which will be able to be applied to drug screening targeting cancer stem cells. On the other hand, since MCF-7 cells migrated in a 3D hydrogel that displayed AG73, we could construct the metastatic model of breast cancer cells, which is helpful for the elucidation of breast cancer cells and drug screening against cancer cells under metastatic state. Therefore, functionalized (FFiK)2 hydrogels with various bioactive sequences can be used to regulate cancer cell function for tumor engineering and drug screening. |
| 巻・号 | 46 |
| ページ | 116345 |
| 公開日 | 2021-9-15 |
| DOI | 10.1016/j.bmc.2021.116345 |
| PII | S0968-0896(21)00353-9 |
| PMID | 34416510 |
| MeSH | Antineoplastic Agents / chemical synthesis Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Breast Neoplasms / drug therapy* Breast Neoplasms / pathology Cell Culture Techniques* Cell Proliferation / drug effects Dose-Response Relationship, Drug Drug Screening Assays, Antitumor Female Humans Hydrogels / chemistry Hydrogels / pharmacology* MCF-7 Cells Macromolecular Substances / chemical synthesis Macromolecular Substances / chemistry Macromolecular Substances / pharmacology Molecular Structure Peptides / chemical synthesis Peptides / chemistry Peptides / pharmacology* Structure-Activity Relationship Tumor Cells, Cultured |
| IF | 3.073 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MCF7(RCB1904) |