Reference - Detail
| RRC ID | 68882 |
|---|---|
| Author | Daikuzono H, Yamazaki M, Sato Y, Takahashi T, Yamagata K. |
| Title | Development of a DELFIA method to detect oncofetal antigen ROR1-positive exosomes. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is highly expressed in a wide variety of hematological and solid cancers, but is low or absent in adult tissues. Here, we show that ROR1 is released with exosomes from ROR1-positive cancer cells. We also developed a simple dissociation-enhanced lanthanide fluorescence immunoassay (DELFIA) to detect cancer-derived ROR1-positive exosomes, which are captured by two anti-ROR1 antibodies and detected by the fluorescence of free chelating europium. This new DELFIA method can detect cancer-derived ROR1-positive exosomes in the cell supernatant and serum with a wide range and rapidly compared with the conventional Western blot assay. This method may be useful as a companion diagnostics for ROR1-positive cancers. |
| Volume | 578 |
| Pages | 170-176 |
| Published | 2021-11-12 |
| DOI | 10.1016/j.bbrc.2021.08.054 |
| PII | S0006-291X(21)01237-7 |
| PMID | 34597914 |
| MeSH | Animals Antigens, Neoplasm / metabolism* Biomarkers, Tumor / blood* Biomarkers, Tumor / metabolism Cell Line, Tumor Disease Models, Animal Exosomes / metabolism Exosomes / pathology* Female Humans Immunoassay / methods* Mice Mice, Inbred BALB C Neoplasms / blood Neoplasms / metabolism Neoplasms / pathology* Receptor Tyrosine Kinase-like Orphan Receptors / metabolism* |
| IF | 2.985 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | DU145(RCB2143) PANC-1(RCB2095) MIA Paca2(RCB2094) |