| RRC ID |
68898
|
| 著者 |
Hamada T, Souda M, Yoshimura T, Sasaguri S, Hatanaka K, Tasaki T, Yoshioka T, Ohi Y, Yamada S, Tsutsui M, Umekita Y, Tanimoto A.
|
| タイトル |
Anti-apoptotic effects of PCP4/PEP19 in human breast cancer cell lines: a novel oncotarget.
|
| ジャーナル |
Oncotarget
|
| Abstract |
The PCP4/PEP19 is a calmodulin-binding anti-apoptotic peptide in neural cells but its potential role in human cancer has largely been unknown. We investigated the expression of PCP4/PEP19 in human breast cancer cell lines MCF-7, SK-BR-3, and MDA-MB-231 cells, and found that estrogen receptor (ER)-positive MCF-7 and ER-negative SK-BR-3 cells expressed PCP4/PEP19. In the MCF-7 cells, cell proliferation was estrogen-dependent, and PCP4/PEP19 expression was induced by estrogen. In both cell lines, PCP4/PEP19 knockdown induced apoptosis and slightly decreased Akt phosphorylation. Knockdown of calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1), resulting in decreased phospho-Akt(Thr308), enhanced apoptosis in SK-BR-3 but not in MCF-7 cells. CaMKK2 knockdown moderately decreased phospho-Akt(Thr308) and increased apoptosis in MCF-7 cells but not in SK-BR-3 cells. These data indicated that PCP4/PEP19 regulates apoptosis but exact mechanism is still unknown. PCP4/PEP19 can therefore potentially serve as independent oncotarget for therapy of PCP4/PEP19-positive breast cancers irrespective of ER expression.
|
| 巻・号 |
5(15)
|
| ページ |
6076-86
|
| 公開日 |
2014-8-15
|
| DOI |
10.18632/oncotarget.2161
|
| PII |
2161
|
| PMID |
25153723
|
| PMC |
PMC4171614
|
| MeSH |
Apoptosis / physiology
Breast Neoplasms / genetics*
Breast Neoplasms / metabolism
Cell Line, Tumor
Cell Proliferation / physiology
Female
Humans
MCF-7 Cells
Molecular Targeted Therapy
Nerve Tissue Proteins / genetics*
Nerve Tissue Proteins / metabolism
Phosphorylation
Signal Transduction
Transfection
|
| IF |
5.168
|
| リソース情報 |
| ヒト・動物細胞 |
MCF7(RCB1904) |
