論文 - 詳細
| RRC ID | 68929 |
|---|---|
| 著者 | Kato S, Shiozaki A, Kudou M, Shimizu H, Kosuga T, Ohashi T, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Konishi E, Otsuji E. |
| タイトル | TRPV2 Promotes Cell Migration and Invasion in Gastric Cancer via the Transforming Growth Factor-β Signaling Pathway. |
| ジャーナル | Ann Surg Oncol |
| Abstract |
BACKGROUND:Transient receptor potential vanilloid 2 (TRPV2) is a highly Ca2+-permeable ion channel that is involved in a number of cellular processes. It is expressed in various human cancers; however, the role of TRPV2 in gastric cancer (GC) remains poorly understood. METHODS:TRPV2 gene expression was knocked down in GC cell lines by small-interfering RNA (siRNA), and the biological roles of TRPV2 in the proliferation, migration, and invasion of GC cells were then investigated. The gene expression profile of GC was elucidated using a microarray analysis. TRPV2 expression in tumor tissue sections was analyzed by immunohistochemistry. RESULTS:The migration and invasion abilities of GC cells were inhibited by the knockdown of TRPV2. Moreover, the microarray assay revealed that TRPV2 was associated with the transforming growth factor (TGF)-β signaling pathway. Immunohistochemical staining showed that the strong expression of TRPV2 correlated with lymphatic invasion, venous invasion, pathological T (pT), pathological N (pN), and a poor prognosis in GC patients. CONCLUSIONS:TRPV2 appeared to promote tumor migration and invasion via the TGF-β signaling pathway, and the strong expression of TRPV2 was associated with a worse prognosis in GC patients. |
| 巻・号 | 29(5) |
| ページ | 2944-2956 |
| 公開日 | 2022-5-1 |
| DOI | 10.1245/s10434-021-11132-5 |
| PII | 10.1245/s10434-021-11132-5 |
| PMID | 34855064 |
| MeSH | Cell Line, Tumor Cell Movement Cell Proliferation Gene Expression Regulation, Neoplastic Humans Neoplasm Invasiveness / genetics RNA, Small Interfering Signal Transduction Stomach Neoplasms* / pathology TRPV Cation Channels / genetics TRPV Cation Channels / metabolism Transforming Growth Factors / genetics Transforming Growth Factors / metabolism |
| IF | 4.061 |
| リソース情報 | |
| ヒト・動物細胞 | HGC-27(RCB0500) NUGC-4(RCB1939) MKN7(RCB0999) MKN45(RCB1001) MKN74(RCB1002) |