論文 - 詳細
| RRC ID | 68936 |
|---|---|
| 著者 | Takahashi T, Zenno S, Ishibashi O, Takizawa T, Saigo K, Ui-Tei K. |
| タイトル | Interactions between the non-seed region of siRNA and RNA-binding RLC/RISC proteins, Ago and TRBP, in mammalian cells. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Small interfering RNA (siRNA)-based RNA interference (RNAi) is widely used for target gene silencing in various organisms. We previously showed that 8-nt-long 5' proximal nucleotides, which include seed sequence (positions 2-8 from the 5' end of guide strand), and the complementary sequence of the passenger strand are capable of being simultaneously replaced with cognate deoxyribonucleotides without any substantial loss of gene silencing. In the present study, examination was made of RNA requirements in the non-seed region of siRNA. The non-seed region of siRNA was found to be subdivided into four domains, in which two nucleotide pairs (positions 13 and 14) were replaceable with cognate deoxyribonucleotides without reducing RNAi activity. However, RNA sequences at positions 9-12 and 15-18 were essential for effective gene silencing, and these two double-stranded RNA cores are required for binding of the trans-activation response RNA-binding protein (TRBP). The terminal RNA (positions 19-21) provided Argonaute protein binding sites. Argonaute binding was enhanced by the presence of RNAs at positions 15-18. Knockdown experiments showed that, unlike Argonaute and TRBP, Dicer was dispensable for RNAi. Based on these observations, we discuss possible RNA/protein and protein/protein interactions in RNA-induced silencing complex formation. |
| 巻・号 | 42(8) |
| ページ | 5256-69 |
| 公開日 | 2014-4-1 |
| DOI | 10.1093/nar/gku153 |
| PII | gku153 |
| PMID | 24561616 |
| PMC | PMC4005638 |
| MeSH | Animals Argonaute Proteins / metabolism* CHO Cells Cricetinae Cricetulus DNA / chemistry HeLa Cells Humans RNA Interference* RNA, Small Interfering / chemistry* RNA, Small Interfering / metabolism RNA-Binding Proteins / metabolism* Ribonuclease III / metabolism |
| IF | 11.502 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1 |