| RRC ID |
68938
|
| Author |
Shimizu K, Iyoda T, Sanpei A, Nakazato H, Okada M, Ueda S, Kato-Murayama M, Murayama K, Shirouzu M, Harada N, Hidaka M, Fujii SI.
|
| Title |
Identification of TCR repertoires in functionally competent cytotoxic T cells cross-reactive to SARS-CoV-2.
|
| Journal |
Commun Biol
|
| Abstract |
SARS-CoV-2-specific CD8+ T cells are scarce but detectable in unexposed healthy donors (UHDs). It remains unclear whether pre-existing human coronavirus (HCoV)-specific CD8+ T cells are converted to functionally competent T cells cross-reactive to SARS-CoV-2. Here, we identified the HLA-A24-high binding, immunodominant epitopes in SARS-CoV-2 spike region that can be recognized by seasonal coronavirus-specific CD8+ T cells from HLA-A24+ UHDs. Cross-reactive CD8+ T cells were clearly reduced in patients with hematological malignancy, who are usually immunosuppressed, compared to those in UHDs. Furthermore, we showed that CD8+ T cells in response to a selected dominant epitope display multifunctionality and cross-functionality across HCoVs in HLA-A24+ donors. Cross-reactivity of T-cell receptors isolated from them exhibited selective diversity at the single-cell level. Taken together, when stimulated well by immunodominant epitopes, selective pre-existing CD8+ T cells with high functional avidity may be cross-reactive against SARS-CoV-2.
|
| Volume |
4(1)
|
| Pages |
1365
|
| Published |
2021-12-2
|
| DOI |
10.1038/s42003-021-02885-6
|
| PII |
10.1038/s42003-021-02885-6
|
| PMID |
34857854
|
| PMC |
PMC8640030
|
| MeSH |
Antigens, Viral / immunology*
CD8-Positive T-Lymphocytes / immunology
COVID-19 / immunology
Cross Reactions
Humans
Immunodominant Epitopes / immunology*
Receptors, Antigen, T-Cell / immunology*
SARS-CoV-2 / immunology*
T-Lymphocytes, Cytotoxic / immunology*
|
| IF |
4.165
|
| Resource |
| Human and Animal Cells |
SKW-3(RCB1168) |
