RRC ID 68945
Author Wu YX, Lin YS, Li SC, Yao X, Cheng M, Zhu L, Liu HY.
Title microRNA-194 is increased in polycystic ovary syndrome granulosa cell and induce KGN cells apoptosis by direct targeting heparin-binding EGF-like growth factor.
Journal Reprod Biol Endocrinol
Abstract BACKGROUND:Polycystic ovary syndrome (PCOS) is an endocrine-related follicular developmental disorder that affects 50 %-70 % of reproductive-aged women diagnosed with ovulation-related infertility. Abnormal proliferation and apoptosis of granulosa cells (GCs) are thought to be the critical factors leading to abnormal maturation of follicles. It has been shown that microRNAs (miRNAs) exert a significant influence in the pathogenesis of PCOS; however, the relationship between miRNA, PCOS, and GC apoptosis is not entirely understood.
METHODS:To clarify the effect of miR-194 in PCOS, CCK-8, Ki67 staining, AO/EB, and flow cytometry assays were used to assess cell growth, proliferation, and apoptosis in KGN cells, which were artificially stimulated to overexpress miR-194. Luciferase reporter assays and rescue experiments were used to elucidate the mechanism underlying miR-194 in PCOS.
RESULTS:miR-194 expression was significantly up-regulated in rat models of PCOS and the ovarian GCs of PCOS patients. miR-194 suppression promoted KGN cell growth and proliferation. miR-194 overexpression also induced cell apoptosis, while miR-194 downregulation had an opposite effect. Furthermore, up-regulating heparin-binding EGF-like growth factor (HB-EGF) expression rescued the pro-apoptotic effects of miR-194 upregulation on KGN cells.
CONCLUSIONS:miR-194 is increased in PCOS granulosa cell and may function as a novel biomarker and therapeutic target for KGN cells via HB-EGF regulation.
Volume 19(1)
Pages 170
Published 2021-11-23
DOI 10.1186/s12958-021-00850-w
PII 10.1186/s12958-021-00850-w
PMID 34814928
PMC PMC8609843
MeSH Adult Animals Apoptosis / physiology* Cell Line Cell Proliferation / physiology Female Granulosa Cell Tumor / metabolism* Granulosa Cell Tumor / pathology Granulosa Cells / metabolism* Granulosa Cells / pathology Heparin-binding EGF-like Growth Factor / biosynthesis* Humans MicroRNAs / biosynthesis* Polycystic Ovary Syndrome / metabolism* Polycystic Ovary Syndrome / pathology Rats Rats, Sprague-Dawley Young Adult
IF 3.235
Human and Animal Cells KGN(RCB1154)