論文 - 詳細
| RRC ID | 68952 |
|---|---|
| 著者 | Endo M, Kim JI, Shioi NA, Iwai S, Kuraoka I. |
| タイトル | Arabidopsis thaliana endonuclease V is a ribonuclease specific for inosine-containing single-stranded RNA. |
| ジャーナル | Open Biol |
| Abstract |
Endonuclease V is highly conserved, both structurally and functionally, from bacteria to humans, and it cleaves the deoxyinosine-containing double-stranded DNA in Escherichia coli, whereas in Homo sapiens it catalyses the inosine-containing single-stranded RNA. Thus, deoxyinosine and inosine are unexpectedly produced by the deamination reactions of adenine in DNA and RNA, respectively. Moreover, adenosine-to-inosine (A-to-I) RNA editing is carried out by adenosine deaminase acting on dsRNA (ADARs). We focused on Arabidopsis thaliana endonuclease V (AtEndoV) activity exhibiting variations in DNA or RNA substrate specificities. Since no ADAR was observed for A-to-I editing in A. thaliana, the possibility of inosine generation by A-to-I editing can be ruled out. Purified AtEndoV protein cleaved the second and third phosphodiester bonds, 3' to inosine in single-strand RNA, at a low reaction temperature of 20-25°C, whereas the AtEndoV (Y100A) protein bearing a mutation in substrate recognition sites did not cleave these bonds. Furthermore, AtEndoV, similar to human EndoV, prefers RNA substrates over DNA substrates, and it could not cleave the inosine-containing double-stranded RNA. Thus, we propose the possibility that AtEndoV functions as an RNA substrate containing inosine induced by RNA damage, and not by A-to-I RNA editing in vivo. |
| 巻・号 | 11(10) |
| ページ | 210148 |
| 公開日 | 2021-10-1 |
| DOI | 10.1098/rsob.210148 |
| PMID | 34665969 |
| PMC | PMC8526164 |
| MeSH | Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Binding Sites Deoxyribonuclease (Pyrimidine Dimer) / genetics Deoxyribonuclease (Pyrimidine Dimer) / metabolism* Gene Expression Regulation, Plant Inosine / chemistry* RNA Editing RNA, Plant / chemistry RNA, Plant / genetics* Substrate Specificity |
| IF | 4.931 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Peer-review(Publons) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda13534 |