RRC ID 68999
Author Zotos D, Quast I, Li-Wai-Suen CSN, McKenzie CI, Robinson MJ, Kan A, Smyth GK, Hodgkin PD, Tarlinton DM.
Title The concerted change in the distribution of cell cycle phases and zone composition in germinal centers is regulated by IL-21.
Journal Nat Commun
Abstract Humoral immune responses require germinal centres (GC) for antibody affinity maturation. Within GC, B cell proliferation and mutation are segregated from affinity-based positive selection in the dark zone (DZ) and light zone (LZ) substructures, respectively. While IL-21 is known to be important in affinity maturation and GC maintenance, here we show it is required for both establishing normal zone representation and preventing the accumulation of cells in the G1 cell cycle stage in the GC LZ. Cell cycle progression of DZ B cells is unaffected by IL-21 availability, as is the zone phenotype of the most highly proliferative GC B cells. Collectively, this study characterises the development of GC zones as a function of time and B cell proliferation and identifies IL-21 as an important regulator of these processes. These data help explain the requirement for IL-21 in normal antibody affinity maturation.
Volume 12(1)
Pages 7160
Published 2021-12-9
DOI 10.1038/s41467-021-27477-0
PII 10.1038/s41467-021-27477-0
PMID 34887406
PMC PMC8660905
MeSH Animals B-Lymphocytes / cytology* B-Lymphocytes / immunology* Cell Cycle* Cell Differentiation* Cell Proliferation Germinal Center / immunology* Interleukins / genetics Interleukins / immunology Mice, Inbred C57BL Mice, Knockout
IF 12.121
Mice RBRC02706 RBRC02707