論文 - 詳細
| RRC ID | 69040 |
|---|---|
| 著者 | Watabe M, Hiraiwa A, Sakai M, Ueno T, Ueno S, Nakajima K, Yaoita Y, Iwao Y. |
| タイトル | Sperm MMP-2 is indispensable for fast electrical block to polyspermy at fertilization in Xenopus tropicalis. |
| ジャーナル | Mol Reprod Dev |
| Abstract |
Sperm matrix metalloproteinase-2 (MMP-2) is necessary for frog fertilization. Monospermy is ensured by a fast, electrical block to polyspermy mediated by a positive fertilization potential. To determine the role of the MMP-2 hemopexin domain (HPX) in a fast block to polyspermy during fertilization of the frog, Xenopus tropicalis, we prepared mutant frogs deficient in mmp2 gene using the transcription activator-like effector nuclease method. mmp2 ΔHPX (-/-) sperm without MMP-2 protein were able to fertilize wild-type (WT; +/+) eggs. However, polyspermy occurred in some eggs. The mutant sperm generated a normal fertilization potential amounting to 10 mV, and were able to fertilize eggs at 10 mV, at which WT sperm never fertilized. Sensitivity during voltage-dependent fertilization decreased in mutant sperm. This study demonstrates for the first time that the genetic alteration of the MMP-2 molecule in sperm causes polyspermy during fertilization of a monospermic species. Our findings provide reliable evidence that sperm MMP-2 is indispensable for the fast, electrical block to polyspermy during Xenopus fertilization. |
| 巻・号 | 88(11) |
| ページ | 744-757 |
| 公開日 | 2021-11-1 |
| DOI | 10.1002/mrd.23540 |
| PMID | 34618381 |
| MeSH | Animals Fertilization* Male Matrix Metalloproteinase 2* / genetics Matrix Metalloproteinase 2* / metabolism Membrane Potentials Ovum Sperm-Ovum Interactions Spermatozoa / metabolism Xenopus laevis |
| IF | 2.823 |
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