| RRC ID |
69040
|
| 著者 |
Watabe M, Hiraiwa A, Sakai M, Ueno T, Ueno S, Nakajima K, Yaoita Y, Iwao Y.
|
| タイトル |
Sperm MMP-2 is indispensable for fast electrical block to polyspermy at fertilization in Xenopus tropicalis.
|
| ジャーナル |
Mol Reprod Dev
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| Abstract |
Sperm matrix metalloproteinase-2 (MMP-2) is necessary for frog fertilization. Monospermy is ensured by a fast, electrical block to polyspermy mediated by a positive fertilization potential. To determine the role of the MMP-2 hemopexin domain (HPX) in a fast block to polyspermy during fertilization of the frog, Xenopus tropicalis, we prepared mutant frogs deficient in mmp2 gene using the transcription activator-like effector nuclease method. mmp2 ΔHPX (-/-) sperm without MMP-2 protein were able to fertilize wild-type (WT; +/+) eggs. However, polyspermy occurred in some eggs. The mutant sperm generated a normal fertilization potential amounting to 10 mV, and were able to fertilize eggs at 10 mV, at which WT sperm never fertilized. Sensitivity during voltage-dependent fertilization decreased in mutant sperm. This study demonstrates for the first time that the genetic alteration of the MMP-2 molecule in sperm causes polyspermy during fertilization of a monospermic species. Our findings provide reliable evidence that sperm MMP-2 is indispensable for the fast, electrical block to polyspermy during Xenopus fertilization.
|
| 巻・号 |
88(11)
|
| ページ |
744-757
|
| 公開日 |
2021-11-1
|
| DOI |
10.1002/mrd.23540
|
| PMID |
34618381
|
| MeSH |
Animals
Fertilization*
Male
Matrix Metalloproteinase 2* / genetics
Matrix Metalloproteinase 2* / metabolism
Membrane Potentials
Ovum
Sperm-Ovum Interactions
Spermatozoa / metabolism
Xenopus laevis
|
| IF |
2.823
|
| リソース情報 |
| ツメガエル・イモリ |
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