RRC ID 69042
著者 Fujitani N, Furukawa J, Araki K, Fujioka T, Takegawa Y, Piao J, Nishioka T, Tamura T, Nikaido T, Ito M, Nakamura Y, Shinohara Y.
タイトル Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers.
ジャーナル Proc Natl Acad Sci U S A
Abstract Although many of the frequently used pluripotency biomarkers are glycoconjugates, a glycoconjugate-based exploration of novel cellular biomarkers has proven difficult due to technical difficulties. This study reports a unique approach for the systematic overview of all major classes of oligosaccharides in the cellular glycome. The proposed method enabled mass spectrometry-based structurally intensive analyses, both qualitatively and quantitatively, of cellular N- and O-linked glycans derived from glycoproteins, glycosaminoglycans, and glycosphingolipids, as well as free oligosaccharides of human embryonic stem cells (hESCs), induced pluripotent stem cells (hiPSCs), and various human cells derived from normal and carcinoma cells. Cellular total glycomes were found to be highly cell specific, demonstrating their utility as unique cellular descriptors. Structures of glycans of all classes specifically observed in hESCs and hiPSCs tended to be immature in general, suggesting the presence of stem cell-specific glycosylation spectra. The current analysis revealed the high similarity of the total cellular glycome between hESCs and hiPSCs, although it was suggested that hESCs are more homogeneous than hiPSCs from a glycomic standpoint. Notably, this study enabled a priori identification of known pluripotency biomarkers such as SSEA-3, -4, and -5 and Tra-1-60/81, as well as a panel of glycans specifically expressed by hESCs and hiPSCs.
巻・号 110(6)
ページ 2105-10
公開日 2013-2-5
DOI 10.1073/pnas.1214233110
PII 1214233110
PMID 23345451
PMC PMC3568377
MeSH Animals Biomarkers / chemistry Biomarkers / metabolism* Carbohydrate Metabolism* Cell Line Cell Line, Tumor Chromatography, High Pressure Liquid Cricetinae Embryonic Stem Cells / metabolism Glycomics / methods* Humans Induced Pluripotent Stem Cells / metabolism Metabolome Molecular Structure Oligosaccharides / chemistry Oligosaccharides / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
IF 9.412
リソース情報
ヒト・動物細胞 HeLa(RCB0007) NEC8(RCB0489) A549(RCB0098) KLM-1(RCB2138) CACO-2(RCB0988) Hep G2(RCB1648) 293(RCB1637) MRC-5(RCB0211)