| RRC ID |
69047
|
| 著者 |
Tsai CR, Wang Y, Jacobson A, Sankoorikkal N, Chirinos JD, Burra S, Makthal N, Kumaraswami M, Galko MJ.
|
| タイトル |
Pvr and distinct downstream signaling factors are required for hemocyte spreading and epidermal wound closure at Drosophila larval wound sites.
|
| ジャーナル |
G3 (Bethesda)
|
| Abstract |
Tissue injury is typically accompanied by inflammation. In Drosophila melanogaster, wound-induced inflammation involves adhesive capture of hemocytes at the wound surface followed by hemocyte spreading to assume a flat, lamellar morphology. The factors that mediate this cell spreading at the wound site are not known. Here, we discover a role for the Platelet-derived growth factor (PDGF)/Vascular endothelial growth factor (VEGF)-related receptor (Pvr) and its ligand, Pvf1, in blood cell spreading at the wound site. Pvr and Pvf1 are required for spreading in vivo and in an in vitro spreading assay where spreading can be directly induced by Pvf1 application or by constitutive Pvr activation. In an effort to identify factors that act downstream of Pvr, we performed a genetic screen in which select candidates were tested to determine if they could suppress the lethality of Pvr overexpression in the larval epidermis. Some of the suppressors identified are required for epidermal wound closure, another Pvr-mediated wound response, some are required for hemocyte spreading in vitro, and some are required for both. One of the downstream factors, Mask, is also required for efficient wound-induced hemocyte spreading in vivo. Our data reveals that Pvr signaling is required for wound responses in hemocytes (cell spreading) and defines distinct downstream signaling factors that are required for either epidermal wound closure or hemocyte spreading.
|
| 巻・号 |
12(1)
|
| 公開日 |
2022-1-4
|
| DOI |
10.1093/g3journal/jkab388
|
| PII |
6423993
|
| PMID |
34751396
|
| PMC |
PMC8728012
|
| MeSH |
Animals
Drosophila*
Drosophila Proteins* / genetics
Drosophila melanogaster / genetics
Egg Proteins / physiology
Epidermis
Hemocytes
Larva / genetics
Receptor Protein-Tyrosine Kinases
Vascular Endothelial Growth Factor A
|
| IF |
2.781
|
| リソース情報 |
| ショウジョウバエ |
9375R-1
7717R-1
1587R-1
6313R-2
8222R-3 |
