論文 - 詳細
| RRC ID | 69071 |
|---|---|
| 著者 | Suruzzaman M, Cao T, Lu J, Wang Y, Su M, Yang M. |
| タイトル | Evaluation of the MIB-producing potential based on real-time qPCR in drinking water reservoirs. |
| ジャーナル | Environ Res |
| Abstract |
Cyanobacteria release 2-methylisoborneol (MIB) as a secondary metabolite. Here, we propose a reverse transcription quantitative real-time PCR (RT-qPCR) based method to evaluate the MIB-producing potential in source water by detecting the MIB-synthesis gene (mic). A MIBQSF/R primer set was designed based on 35 mic gene sequences obtained from 12 pure-cultured MIB-producing strains and 23 sequences from the NCBI database. This primer set successfully identified all known 43 MIB-producing cyanobacterial strains (12 from this study and 31 from the NCBI database), belonging to different genera, showing a wider coverage than previous primer sets. The efficiency of the method was proved by the amplification efficiency (E = 91.23%), R2 of the standard curve (0.999), the limit of detection (LOD, 5.7 fg μL-1), and the limit of quantification (LOQ, 1.86 × 104 gene copies μL-1). Further, the method was verified by the correlation between the mic gene abundance and MIB concentration 50 field samples from different reservoirs (R2 = 0.614, p < 0.001) and one reservoir (R2 = 0.752, p < 0.001), suggesting its potential as an alternative warning tool to evaluate the risk of MIB problems in source water. |
| 巻・号 | 204(Pt C) |
| ページ | 112308 |
| 公開日 | 2022-3-1 |
| DOI | 10.1016/j.envres.2021.112308 |
| PII | S0013-9351(21)01609-1 |
| PMID | 34757030 |
| MeSH | Camphanes / analysis Cyanobacteria* / genetics Cyanobacteria* / metabolism Drinking Water* / analysis Odorants / analysis Real-Time Polymerase Chain Reaction |
| IF | 5.715 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-512 NIES-4062 NIES-4063 NIES-4237 NIES-4238 |