RRC ID 69076
著者 Kawai J, Kanazawa M, Suzuki R, Kikuchi N, Hayakawa Y, Sekimoto H.
タイトル Highly efficient transformation of the model zygnematophycean alga Closterium peracerosum-strigosum-littorale complex by square-pulse electroporation.
ジャーナル New Phytol
Abstract The zygnematophycean algae occupy an important phylogenetic position as the closest living relatives of land plants. Reverse genetics is quite useful for dissecting the functions of genes. However, this strategy requires genetic transformation, and there are only a few reports of successful transformation in zygnematophycean algae. Here, we established a simple and highly efficient transformation technique for the unicellular zygnematophycean alga Closterium peracerosum-strigosum-littorale complex using a square electric pulse-generating electroporator without the need for cell wall removal. Using this method, the transformation efficiency increased > 100-fold compared with our previous study using particle bombardment. We also succeeded in performing CRISPR/Cas9-based gene knockout using this new method. Our method requires only small amounts of labor, time and incubator space. Moreover, our technique could also be utilized to transform other charophycean algae with available genome information by optimizing the electric pulse conditions.
巻・号 233(1)
ページ 569-578
公開日 2022-1-1
DOI 10.1111/nph.17763
PMID 34605030
MeSH Closterium* Electroporation Phylogeny Plants Transformation, Genetic
IF 8.512
リソース情報
藻類 NIES-67 NIES-68