| RRC ID |
69132
|
| 著者 |
Komatsu M, Tsukahara H, Bai H, Takahashi M, Wakai T, Kawahara M.
|
| タイトル |
Cell-cycle dependent GATA2 subcellular localization in mouse 2-cell embryos.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
GATA factors are essential transcription factors for embryonic development that broadly control the transcription of other genes. This study aimed to examine GATA2 protein localization in mouse embryos at the 2-cell stage, when drastic transformation in gene expression occurs for subsequent development in early embryos. We first analyzed GATA2 localization in 2-cell embryos at the interphase and mitotic phases by immunofluorescence analysis. In the interphase, GATA2 protein was localized in the nucleus, as a common transcription factor. In the mitotic phase, GATA2 protein was observed as a focally-aggregated spot around the nucleus of each blastomere. To explore the relationship between GATA2 protein localization and cell cycle progression in mouse 2-cell stage embryos, GFP-labeled GATA2 protein was overexpressed in the blastomere of 2-cell embryos. Overexpression of GFP-labeled GATA2 protein arrested cellular mitosis, focally aggregated GATA2 protein expression was not observed. This mitotic arrest by GATA2 overexpression was not accompanied with the upregulation of a 2-cell stage specific gene, murine endogenous retrovirus-L. These results suggest that GATA2 protein localization changes dynamically depending on cell cycle progression in mouse 2-cell embryos; in particular, focally aggregated localization of GATA2 in the mitotic phase requires appropriate cell cycle progression.
|
| 巻・号 |
584
|
| ページ |
1-6
|
| 公開日 |
2021-12-20
|
| DOI |
10.1016/j.bbrc.2021.10.077
|
| PII |
S0006-291X(21)01501-1
|
| PMID |
34741809
|
| MeSH |
Animals
Blastocyst / cytology
Blastocyst / metabolism*
Blastomeres / cytology
Blastomeres / metabolism
Cell Cycle / genetics*
Cell Nucleus / genetics*
Cell Nucleus / metabolism
Female
GATA2 Transcription Factor / genetics*
GATA2 Transcription Factor / metabolism
Gene Expression Regulation, Developmental*
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Interphase / genetics
Mice, Inbred ICR
Microscopy, Fluorescence
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
|
| IF |
2.985
|
| リソース情報 |
| 遺伝子材料 |
CS-CA-GFP (RDB05964) |
