論文 - 詳細
| RRC ID | 69132 |
|---|---|
| 著者 | Komatsu M, Tsukahara H, Bai H, Takahashi M, Wakai T, Kawahara M. |
| タイトル | Cell-cycle dependent GATA2 subcellular localization in mouse 2-cell embryos. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
GATA factors are essential transcription factors for embryonic development that broadly control the transcription of other genes. This study aimed to examine GATA2 protein localization in mouse embryos at the 2-cell stage, when drastic transformation in gene expression occurs for subsequent development in early embryos. We first analyzed GATA2 localization in 2-cell embryos at the interphase and mitotic phases by immunofluorescence analysis. In the interphase, GATA2 protein was localized in the nucleus, as a common transcription factor. In the mitotic phase, GATA2 protein was observed as a focally-aggregated spot around the nucleus of each blastomere. To explore the relationship between GATA2 protein localization and cell cycle progression in mouse 2-cell stage embryos, GFP-labeled GATA2 protein was overexpressed in the blastomere of 2-cell embryos. Overexpression of GFP-labeled GATA2 protein arrested cellular mitosis, focally aggregated GATA2 protein expression was not observed. This mitotic arrest by GATA2 overexpression was not accompanied with the upregulation of a 2-cell stage specific gene, murine endogenous retrovirus-L. These results suggest that GATA2 protein localization changes dynamically depending on cell cycle progression in mouse 2-cell embryos; in particular, focally aggregated localization of GATA2 in the mitotic phase requires appropriate cell cycle progression. |
| 巻・号 | 584 |
| ページ | 1-6 |
| 公開日 | 2021-12-20 |
| DOI | 10.1016/j.bbrc.2021.10.077 |
| PII | S0006-291X(21)01501-1 |
| PMID | 34741809 |
| MeSH | Animals Blastocyst / cytology Blastocyst / metabolism* Blastomeres / cytology Blastomeres / metabolism Cell Cycle / genetics* Cell Nucleus / genetics* Cell Nucleus / metabolism Female GATA2 Transcription Factor / genetics* GATA2 Transcription Factor / metabolism Gene Expression Regulation, Developmental* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Interphase / genetics Mice, Inbred ICR Microscopy, Fluorescence Reverse Transcriptase Polymerase Chain Reaction Time Factors |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CS-CA-GFP (RDB05964) |